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CD8 anticorps (APC)

CD8 Reactivité: Humain FACS, IF Hôte: Souris Monoclonal MEM-31 APC
N° du produit ABIN1112229
  • Antigène Voir toutes CD8 Anticorps
    CD8
    Reactivité
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    Humain
    Hôte
    • 243
    • 54
    • 39
    Souris
    Clonalité
    • 280
    • 47
    • 2
    Monoclonal
    Conjugué
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    Cet anticorp CD8 est conjugé à/à la APC
    Application
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    Flow Cytometry (FACS), Immunofluorescence (IF)
    Attributs du produit
    Monoclonal Mouse Anti-Human CD8 APC, is recommended for use in flow cytometry for identification of human T cells suppressor/cytotoxic expressing the 32 kDa M.W. surface antigen.
    Clone
    MEM-31
    Isotype
    IgG2a
    Top Product
    Discover our top product CD8 Anticorps primaire
  • Indications d'application
    It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
    Commentaires

    Allophycocyanin (Febico, Far East Bio-Tech Co.).

    Prélèvement de l'échantillon
    1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD8 APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
    Stock
    4 °C
  • Lyle, Christofidou-Solomidou, Liu, Elder, Albelda, Cotsarelis: "Human hair follicle bulge cells are biochemically distinct and possess an epithelial stem cell phenotype." dans: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, Vol. 4, Issue 3, pp. 296-301, (2000) (PubMed).

    Nuckols, Shea, Horenstein, Burchette, Prieto: "Quantitation of intraepidermal T-cell subsets in formalin-fixed, paraffin-embedded tissue helps in the diagnosis of mycosis fungoides." dans: Journal of cutaneous pathology, Vol. 26, Issue 4, pp. 169-75, (1999) (PubMed).

    Yamagata, Tanaka, Kudo: "A quantitative immunohistochemical evaluation of inflammatory cells at the affected and unaffected sites of inflammatory bowel disease." dans: Journal of gastroenterology and hepatology, Vol. 13, Issue 8, pp. 801-8, (1998) (PubMed).

  • Antigène
    CD8
    Autre désignation
    CD8 (CD8 Produits)
    Synonymes
    anticorps CD8, anticorps Leu2, anticorps MAL, anticorps p32, anticorps CD8a molecule, anticorps CD8A
    Sujet
    30/32 kDa MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD8 cluster on a sub-population of peripheral blood T lymphocytes, 60% of thymocytes, and a limited number of malignancies of T cell origin. Normal B lymphocytes, monocytes or granulocytes do not express surface CD8 antigen.
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