CD35 anticorps

N° du produit ABIN111245

Détail du produit anti-CD35 anticorps

Antigène: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD35 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Réactivité croisée (Details): Species reactivity (tested):Human.

Immunogène: Purified C3b receptor from Human red blood cells.

Clone: J3D3

Isotype: IgG1

Information d'application

Indications d'application: Mechanism of degradation of C3b and Analysis of Lymphoid and Myeloid differentiation: Clone J3D3 is a blocking antibody. It inhibits CR1 mediated decay of cells and blocks C3bdependent rosette formation with Lymphocytes. Fluorescence Microscopy or Flow Cytometry: 2 μg/5x10e5 cells/test. Immunohistochemistry: 1/25-1/50. Suitable on Frozen sections or cell smears, and onBouin's fixed, Paraffin-embedded tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL of distilled water.

Concentration: 0.2 mg/mL (prior to lyophilization)

Buffer: PBS containing 1 mg/mL BSA as stabilizer and no preservative.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 4-8 °C for one month or at -20 °C for longer.

Références pour anticorps anti-CD35 (ABIN111245)

Schwartz, Barker, Long, Kaufman, McCracken, Allen: "Natural IgM mediates complement-dependent uptake of Francisella tularensis by human neutrophils via complement receptors 1 and 3 in nonimmune serum." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 3064-77, (2012) (PubMed).

Molnar: "Glycoproteins of Ehrlich ascites carcinoma cells. Incorporation of [14C]glucosamine and [14C]sialic acid into membrane proteins." dans: Biochemistry, Vol. 6, Issue 10, pp. 3064-76 (PubMed).

Détails sur CD35

Antigène: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Autre désignation: CD35 / C3BR (CR1 Produits)

Synonymes: anticorps C3BR, anticorps C4BR, anticorps CD35, anticorps KN, anticorps C3DR, anticorps CD21, anticorps Cr-1, anticorps Cr-2, anticorps Cr1, anticorps CR1-L, anticorps CR2, anticorps CR1, anticorps complement C3b/C4b receptor 1 (Knops blood group), anticorps complement receptor 2, anticorps complement component (3d/Epstein Barr virus) receptor 2, anticorps complement receptor type 1, anticorps CR1, anticorps Cr2, anticorps CR2, anticorps LOC718120

Sujet: This gene encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.Synonyms: C3b/C4b receptor, CR1, Complement receptor type 1

ID gène: 9606

UniProt: P17927

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity