F4/80 anticorps

N° du produit ABIN111845

Détail du produit anti-F4/80 anticorps

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp F4/80 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This antibody is useful for the detection of the F4/80 antigen on major subpopulations of resident tissue macrophages. The antigen expression increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. This clone is the only Macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model. Antigen Distribution Tissue Sections: The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells. Isolated Cells: expressed in vitro on over 80 % of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes.Antigen Distribution

Réactivité croisée (Details): Species reactivity (tested):Mouse. Also stains Human Heart Macrophages.

Purification: Affinity Chromatography

Immunogène: Cultured Macrophages. Remarks: The antigen/epitope is a 125 kDa extracellular membrane protein sensitive to 2-Mercaptoetanol.

Clone: BM8

Isotype: IgG2a

Information d'application

Indications d'application: Immunohistochemistry on Frozen Sections: 0.4 μg/mL (1/500). Immunohistochemistry on Paraffin Sections: 4 μg/mL (1/50). Proteinase K pretreatment forantigen retrieval is recommended. Suggested Positive Control: Mouse spleen. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with Frozen, ice-cold Acetone-Fixed Sections: (The whole procedure is performed at room temperature)1. Wash in PBS

Restrictions: For Research Use only

Stockage

Reconstitution: Restore by adding 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: Stock Solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.01 % Kathon as a preservative

Agent conservateur: Kathon CG

Précaution d'utilisation: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-F4/80 (ABIN111845)

Braunger, Leimbeck, Schlecht, Volz, Jägle, Tamm: "Deletion of ocular transforming growth factor ? signaling mimics essential characteristics of diabetic retinopathy." dans: The American journal of pathology, Vol. 185, Issue 6, pp. 1749-68, (2015) (PubMed).

Lemmermann, Krmpotic, Podlech, Brizic, Prager, Adler, Karbach, Wu, Jonjic, Reddehase, Adler: "Non-redundant and redundant roles of cytomegalovirus gH/gL complexes in host organ entry and intra-tissue spread." dans: PLoS pathogens, Vol. 11, Issue 2, pp. e1004640, (2015) (PubMed).

Ilves, Palomäki, Vippola, Lehto, Savolainen, Savinko, Alenius: "Topically applied ZnO nanoparticles suppress allergen induced skin inflammation but induce vigorous IgE production in the atopic dermatitis mouse model." dans: Particle and fibre toxicology, Vol. 11, pp. 38, (2014) (PubMed).

Stangl, Gehrmann, Riegger, Kuhs, Riederer, Sievert, Hube, Mocikat, Dressel, Kremmer, Pockley, Friedrich, Vigh, Skerra, Multhoff: "Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 2, pp. 733-8, (2011) (PubMed).

Spyridonidou, Fousteris, Antonia, Chatzianastasiou, Papapetropoulos, Nikolaropoulos: "Tricyclic indole and dihydroindole derivatives as new inhibitors of soluble guanylate cyclase." dans: Bioorganic & medicinal chemistry letters, Vol. 19, Issue 16, pp. 4810-3, (2009) (PubMed).

Kimura, Miyashita, Suzuki, Kobayashi, Watanabe, Sonoda, Ohta, Fujiwara, Shimosegawa, Sato: "Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2 in the regulation of angiogenesis." dans: Blood, Vol. 113, Issue 19, pp. 4810-8, (2009) (PubMed).

Kakehashi, Nishioku, Tsukuba, Kadowaki, Nakamura, Yamamoto: "Differential regulation of the nature and functions of dendritic cells and macrophages by cathepsin E." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 9, pp. 5728-37, (2007) (PubMed).

Torzewski, Shaw, Han, Shortal, Lackner, Witztum, Palinski, Tsimikas: "Reduced in vivo aortic uptake of radiolabeled oxidation-specific antibodies reflects changes in plaque composition consistent with plaque stabilization." dans: Arteriosclerosis, thrombosis, and vascular biology, Vol. 24, Issue 12, pp. 2307-12, (2004) (PubMed).

Détails sur F4/80

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Autre désignation: Macrophage F4/80 Antigen (EMR1 Produits)

Synonymes: anticorps EMR1, anticorps TM7LN3, anticorps DD7A5-7, anticorps EGF-TM7, anticorps F4/80, anticorps Gpf480, anticorps Ly71, anticorps adhesion G protein-coupled receptor E1, anticorps ADGRE1, anticorps LOC100424335, anticorps Adgre1

Sujet: F4/80 protein binds to Macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. F4/80 is not expressed by lymphocytes or polymorphonuclear cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480

ID gène: 13733

NCBI Accession: NP_034260

UniProt: Q61549