F4/80 anticorps (PE)

N° du produit ABIN111857

Détail du produit anti-F4/80 anticorps (PE)

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp F4/80 est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Specificité: This antibody recognises the F4/80 antigen. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (5). We recommende the use of Cat.-No BM4008LE for this purpose.

Réactivité croisée (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein A

Immunogène: Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice. Remarks: Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS-1 mouse myeloma cell line.

Clone: CI:A3-1

Isotype: IgG2b

Information d'application

Indications d'application: Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL distilled water.

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA, 5 % Sucrose

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Détails sur F4/80

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Autre désignation: Macrophage F4/80 Antigen (EMR1 Produits)

Synonymes: anticorps EMR1, anticorps TM7LN3, anticorps DD7A5-7, anticorps EGF-TM7, anticorps F4/80, anticorps Gpf480, anticorps Ly71, anticorps adhesion G protein-coupled receptor E1, anticorps ADGRE1, anticorps LOC100424335, anticorps Adgre1

Sujet: Murine F4/80 antigen is a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with Human EGF module-containing mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480

ID gène: 13733

NCBI Accession: NP_034260

UniProt: Q61549