CD93 anticorps (Biotin)

N° du produit ABIN111896

Détail du produit anti-CD93 anticorps (Biotin)

Antigène: CD93

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD93 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This Monoclonal antibody clone X-2 recognizes a 110 kDa O-sialoglycoprotein on granulocytes, monocytes and endothelial cells. Upon differentiation of cultivated monocytes into macrophages, CDw93 ceases to be expressed on day 5-6, which makes it a very useful marker to differentiate those two cell populations. Antigen Distribution Isolated Cells: Positive with all granulocytes, with staining increasing transiently upon maturation. Antigen expression is increased by TNFα, GM-CSF, (fMLP) treatment. No effect on superoxide production by binding to neutrophils has been noted. PMA stimulation causes down regulation of the antigen. Positive with monocytes following fMLP, TNF, GM-CSF treatment. Antigen expression decreases on cultured monocytes. Positive on AML cases with monocytic component. Positive on cell lines HL-60, Namalva, K562, THP-1, U937, negative on HMC-1 (human mast cell line). Tissue Sections: The Monoclonal antibody X-2 stains epitheloid cells and Rosai-Dorfman cells and most endothelial cells in the synovial membrane of RA patients.

Purification: Affinity Chromatography

Immunogène: Human monocytes. Remarks: The antigen has been assigned to the CDw93 cluster, the epitope has not been further characterized.

Clone: X-2

Isotype: IgG1

Information d'application

Indications d'application: Suitable for Immunohistochemistry (IHC) on: Frozen sections: 0.5 μg/mL (1/400)Paraffin sections: does not react on routinely processedparaffin sections. It works alos in FACS. Suggested positive control: human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, 0.01 % Thimerosal, 10 mg/mL BSA

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody at 2-8 °C before reconstitution. After reconstitution store at 2-8° for up to 2 months. DO NOT FREEZE. Recontstituted product is stable for 2 days at RT.

Références pour anticorps anti-CD93 (Biotin) (ABIN111896)

Sumide, Matsuoka, Kawamura, Nakatsuka, Fujioka, Asano, Takihara, Sonoda: "A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells." dans: Nature communications, Vol. 9, Issue 1, pp. 2202, (2018) (PubMed).

Détails sur CD93

Antigène: CD93

Autre désignation: CD93 / C1QR1 (CD93 Produits)

Synonymes: anticorps 6030404G09Rik, anticorps AA145088, anticorps AA4.1, anticorps AW555904, anticorps C1qr1, anticorps C1qrp, anticorps Ly68, anticorps C1QR1, anticorps C1qR(P), anticorps C1qRP, anticorps CDw93, anticorps ECSM3, anticorps MXRA4, anticorps dJ737E23.1, anticorps C1qRp, anticorps CD93 antigen, anticorps CD93 molecule, anticorps Cd93, anticorps CD93

Sujet: CD93 a cell-surface glycoprotein and type I membrane protein involve in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.Synonyms: C1q receptor, C1q/MBL/SPA receptor, CDw93, Complement component 1 q subcomponent receptor 1, Complement component C1q receptor, MBL receptor, MXRA4, Matrix-remodeling-associated protein 4, SPA receptor

ID gène: 22918

NCBI Accession: NP_036204

UniProt: Q9NPY3