Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Iba1 anticorps

IBA1 Reactivité: Humain, Rat IHC (p) Hôte: Souris Monoclonal 1022-5 unconjugated
N° du produit ABIN112009
  • Antigène Voir toutes Iba1 (IBA1) Anticorps
    Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
    Reactivité
    • 88
    • 55
    • 47
    • 2
    • 1
    Humain, Rat
    Hôte
    • 77
    • 31
    • 6
    • 1
    Souris
    Clonalité
    • 79
    • 36
    Monoclonal
    Conjugué
    • 55
    • 18
    • 13
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Iba1 est non-conjugé
    Application
    • 80
    • 47
    • 43
    • 22
    • 20
    • 14
    • 14
    • 13
    • 12
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificité
    The antibody reacts with AIF-1.
    Réactivité croisée (Details)
    Species reactivity (tested):Human and Rat.
    Purification
    Affinity Chromatography.
    Immunogène
    Purified Human recombinant AIF-1. Epitope not further characterized.
    Clone
    1022-5
    Isotype
    IgG2b
    Top Product
    Discover our top product IBA1 Anticorps primaire
  • Indications d'application
    Immunohistochemistry on Paraffin Sctions: 10-20 μg/mL (1/20-1/0). Mcrowavepretreatment for antigen retrieval recommended. Suggested positive control: Human spleen. Does not react on routinely processed Frozen Sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocole
    Immunohistochemitry With Unlabeled Monoclonal Antibodies On Paraffin-EmbeddedTissue Sections1) Deparaffinize slides in chloroform for 30min and rehydrate to distilled water2) Antigen retrieval: microwave treatment (700W) for 15min in 300ml 0. 01M citrate bufferpH 6. 0. 3) Incubate slides for 15min in 200ml methanol/4. 8ml 30%H2O24) Rinse slides 2x in distilled water and for 5min in TBS, pH 7. 55) Block with normal swine serum diluted 1: 10 in Tris buffered saline pH 7. 5, containing0. 1% bovine serum albumin (BSA). Cover sections with NSS and incubate for 15min in ahumid chamber at room temperature (RT). 6) Remove serum from sections (do not rinse)7) Incubate with primary antibody diluted in TBS/0. 1%BSA, pH 7. 5 for 60min in a humidchamber at room temperature8) Rinse sections in TBS, pH 7. 5 for 5min, 2x9) Incubate with secondary antibody (rabbit anti mouse IgG+IgM-biotin) 1: 400 diluted inTBS/0. 1%BSA, pH 7. 5 for 30min in a moist chamber, room temperature. Preincubate nowStreptABComplex /HRPO in TBS/0. 1%BSA, pH 7. 5! 10) Rinse sections in TBS, pH 7. 5 for 5min, 2x11) Incubate with StreptABComplex /HRPO for 30min in a humid chamber at roomtemperature12) Rinse sections in TBS, pH 7. 5 for 5min, 2x13) DAB-staining Prepare 3,3' - diaminobenzidintetrahydrochloride (DAB) according themanufacturers instructions (e. g. DAB tablets) and incubate slides for 1-15 min. Check colorreaction to avoid overstaining. 14) Rinse sections in TBS, pH 7. 5 for 5min, 2x15) Counterstain in haematoxylin for 1min and rinse in cold tap water for 10min16) Mount slides with coverslips
    Restrictions
    For Research Use only
  • Reconstitution
    Restore by adding 0.5 mL distilled water.
    Concentration
    0.4 mg/mL
    Buffer
    PBS, pH 7.2, containing 5 mg/mL BSA as stabilizer and 0.01 % Kathon as preservative.
    Agent conservateur
    Kathon CG
    Précaution d'utilisation
    This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène
    Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
    Autre désignation
    AIF1 (IBA1 Produits)
    Synonymes
    anticorps AIF-1, anticorps IBA1, anticorps IRT-1, anticorps IRT1, anticorps AI607846, anticorps D17H6S50E, anticorps G1, anticorps Iba1, anticorps Bart1, anticorps iba1, anticorps mrf-1, anticorps fa04b11, anticorps wu:fa04b11, anticorps AIF, anticorps AIF1, anticorps aif1, anticorps allograft inflammatory factor 1, anticorps AIF1, anticorps Aif1, anticorps aif1
    Sujet
    Allograft Inflammatory Factor-1 is a Ca2+-binding peptide produced by activated macrophages and microglial cells. It has been suggested that AIF-1 expression is associated with chronic inflammatory processes. AIF-1, like the Ca2+-binding peptides MRP8 and MRP14, is expressed by activated macrophages and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts. The recently described apoptosis-inducing factor AIF is not related to AIF-1.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1
    ID gène
    199
    NCBI Accession
    NP_001614
    UniProt
    P55008
    Pathways
    Smooth Muscle Cell Migration
Support technique