GFP anticorps (Texas Red (TR))

N° du produit ABIN116751

Détail du produit anti-GFP anticorps (Texas Red (TR))

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est conjugé à/à la Texas Red (TR)

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.

Attributs du produit: Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 4.2 moles Texas Red per mole of Goat IgG.

Purification: Immunoaffinity Chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène: GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Information d'application

Indications d'application: Designed to detect GFP and its variants in ELISA (sandwich or capture), Immunoblottingand Immunoprecipitation. Monoclonal and polyclonal forms of anti-GFP assayed by ELISA for direct binding of antigenrecognize wild type, recombinant and enhanced forms of GFP. Monoclonal and polyclonal forms anti-GFP assayed in a sandwich ELISA are well suited to

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 witn 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Références pour anticorps anti-GFP (Texas Red (TR)) (ABIN116751)

Koshkin, Ailles, Liu, Krylov: "Metabolic Suppression of a Drug-Resistant Subpopulation in Cancer Spheroid Cells." dans: Journal of cellular biochemistry, Vol. 117, Issue 1, pp. 59-65, (2016) (PubMed).

Martínez-Vega, Garrido, Partearroyo, Cediel, Zeisel, Martínez-Álvarez, Varela-Moreiras, Varela-Nieto, Pajares: "Folic acid deficiency induces premature hearing loss through mechanisms involving cochlear oxidative stress and impairment of homocysteine metabolism." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 2, pp. 418-32, (2015) (PubMed).

Taheri, Saberi Nik, Seyed, Doustdari, Etemadzadeh, Torkashvand, Rafati: "Generation of stable L. major(+EGFP-LUC) and simultaneous comparison between EGFP and luciferase sensitivity." dans: Experimental parasitology, Vol. 150, pp. 44-55, (2015) (PubMed).

Niv, Keiner, Krishna, Witte, Lie, Redecker: "Aberrant neurogenesis after stroke: a retroviral cell labeling study." dans: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2468-75, (2012) (PubMed).

Carvalho, Walter, Baermann-Stapel, Weller, Panne, Schenk, Schneider: "Non-invasive monitoring of immunization progress in mice via IgG from feces." dans: In vivo (Athens, Greece), Vol. 26, Issue 1, pp. 63-9, (2012) (PubMed).

Kokk, Kuuslahti, Keisala, Purmonen, Kaipia, Tammela, Orro, Simovart, Pöllänen: "Expression of luteinizing hormone receptors in the mouse penis." dans: Journal of andrology, Vol. 32, Issue 1, pp. 49-54, (2011) (PubMed).

Eliyahu, Pnueli, Melamed, Scherrer, Gerber, Pines, Rapaport, Arava: "Tom20 mediates localization of mRNAs to mitochondria in a translation-dependent manner." dans: Molecular and cellular biology, Vol. 30, Issue 1, pp. 284-94, (2010) (PubMed).

Suzuki, Mogami, Ihara, Urano: "Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells." dans: Blood, Vol. 113, Issue 2, pp. 470-8, (2009) (PubMed).

van den Borne, Isobe, Verjans, Petrov, Lovhaug, Li, Zandbergen, Ni, Frederik, Zhou, Arbo, Rogstad, Cuthbertson, Chettibi, Reutelingsperger, Blankesteijn, Smits, Daemen, Zannad, Vannan, Narula, Pitt et al.: "Molecular imaging of interstitial alterations in remodeling myocardium after myocardial infarction. ..." dans: Journal of the American College of Cardiology, Vol. 52, Issue 24, pp. 2017-28, (2009) (PubMed).

Wang, Zhang, Wei, Zhang, Zhang, Zhu, Zou, Wang, Mou, Ni, Wu: "Lewis X oligosaccharides targeting to DC-SIGN enhanced antigen-specific immune response." dans: Immunology, Vol. 121, Issue 2, pp. 174-82, (2007) (PubMed).

Robenek, Hofnagel, Buers, Lorkowski, Schnoor, Robenek, Heid, Troyer, Severs: "Butyrophilin controls milk fat globule secretion." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 27, pp. 10385-10390, (2006) (PubMed).

von Rhein, Hunfeld, Ludwig: "Serologic evidence for effective production of cytolysin A in Salmonella enterica serovars typhi and paratyphi A during human infection." dans: Infection and immunity, Vol. 74, Issue 11, pp. 6505-8, (2006) (PubMed).

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP (GFP Produits)

Synonymes: anticorps green fluorescent protein, anticorps gfp

Classe de substances: Viral Protein

Sujet: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein

ID gène: 6100

UniProt: P42212