Chèvre anti-Furet IgG (Chain gamma) Anticorps (Biotin) - Preadsorbed

N° du produit ABIN117100

Détail du produit

Antigène: IgG

Épitope: Chain gamma

Reactivité: Furet

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunomicroscopy (IM)

Attributs du produit: Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.

Purification: Preadsorption: Solid phase absorption

Immunogène: Ferret IgG gamma chain.

Information d'application

Indications d'application: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 μg of Ferret IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1:5,000 to 1:20,000 of the concentration is suggested for thisproduct.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) sodium azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Référence

Sikkema, Begeman, Janssen, Wolters, Geurtsvankessel, de Bruin, Hakze-van der Honing, Eblé, van der Poel, van den Brand, Slaterus, La Haye, Koopmans, Velkers, Kuiken: "Risks of SARS-CoV-2 transmission between free-ranging animals and captive mink in the Netherlands." dans: Transboundary and emerging diseases, Vol. 69, Issue 6, pp. 3339-3349, (2023) (PubMed).

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet: Synonyms: Ferret Immunoglobulin G