ZAP70 anticorps (pTyr319) (PerCP-Cy5.5)

N° du produit ABIN1176924

Détail du produit anti-ZAP70 anticorps (PerCP-Cy5.5)

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Épitope: pTyr319

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ZAP70 est conjugé à/à la PerCP-Cy5.5

Application: Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human phosphorylated ZAP70 Peptide

Clone: 17A-P

Isotype: IgG1

Information d'application

Volume d'échantillon: 5 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed.

Références pour anticorps anti-ZAP70 (PerCP-Cy5.5) (ABIN1176924)

Di Bartolo, Mège, Germain, Pelosi, Dufour, Michel, Magistrelli, Isacchi, Acuto: "Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling." dans: The Journal of biological chemistry, Vol. 274, Issue 10, pp. 6285-94, (1999) (PubMed).

Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." dans: Cell, Vol. 76, Issue 5, pp. 947-58, (1994) (PubMed).

Chan, Kadlecek, Elder, Filipovich, Kuo, Iwashima, Parslow, Weiss: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." dans: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, (1994) (PubMed).

Détails sur ZAP70

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Autre désignation: ZAP70 (ZAP70 Produits)

Synonymes: anticorps SRK, anticorps STCD, anticorps STD, anticorps TZK, anticorps ZAP-70, anticorps 70kDa, anticorps AI327364, anticorps Srk, anticorps mrtle, anticorps mur, anticorps zap70, anticorps MGC80670, anticorps MGC88962, anticorps ZAP70, anticorps zgc:110383, anticorps zeta chain of T-cell receptor associated protein kinase 70, anticorps putative tyrosine-protein kinase zap-70, anticorps zeta-chain (TCR) associated protein kinase, anticorps zeta chain of T cell receptor associated protein kinase 70, anticorps zeta-chain (TCR) associated protein kinase 70kDa, anticorps zeta chain of T cell receptor associated protein kinase 70kDa L homeolog, anticorps zeta chain of T cell receptor associated protein kinase 70kDa, anticorps tyrosine-protein kinase ZAP-70, anticorps ZAP70, anticorps Smp_149470, anticorps Zap70, anticorps zap70.L, anticorps zap70, anticorps LOC707555, anticorps LOC100342021, anticorps LOC100390922, anticorps LOC100542877

Sujet: ZAP70 is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both ZAP70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. TCR stimulation leads to autophosphorylation of ZAP70 at Tyr-315 amd Tyr-319, and mutation of the Tyr-319 site dramatically impairs TCR signaling. In addition, TCR-mediated Lck activity leads to phosphorylation of ZAP70 on Tyr-493 in the regulatory loop of the kinase domain leading to upregulation of ZAP70 kinase activity. The significance of ZAP70 activation in mediating TCR signal transduction has been confirmed by showing that ZAP70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP70 kinase domain which affect the stability of the protein and TCR signaling. Clone 17A/P-ZAP70 recognizes the phosphorylated form of ZAP70 (Y319). It also cross-reacts with SYK (Y352) due to homology of the phosphorylation site with ZAP70 (Y319). The PE-conjugated format has been evaluated using human and mouse model systems. The unconjugated form of the antibody (Cat. No. 612574) has also been shown to work in western blot analysis on human, mouse, and rat cells.
Synonyms: ZAP-70, SRK, STD, TZK, Zeta-chain associated protein kinase, 70kD

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway