IL1R1 anticorps (Interleukin 1 Receptor, Type I)

Details for Product anti-IL1R1 Antibody No. ABIN1176949
Antigène
  • CD121A
  • D2S1473
  • IL-1R-alpha
  • IL1R
  • IL1RA
  • P80
  • CD121a
  • CD121b
  • IL-iR
  • Il1r-1
  • interleukin 1 receptor type 1
  • interleukin 1 receptor, type I
  • IL1R1
  • Il1r1
Reactivité
Souris
165
88
51
2
2
2
2
1
1
1
1
Hôte
Rat
160
32
31
9
3
2
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp IL1R1 est non-conjugé
19
10
8
8
7
5
3
3
2
2
2
2
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
151
109
69
36
26
17
15
14
11
8
5
4
4
2
2
2
2
2
2
1
1
1
Options
Marque BD Pharmingen™
Immunogène IL-1 receptors from the C57BL/6 mouse T lymphoma EL4.IL2
Clone 35F5
Isotype IgG1, kappa
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Stérilité 0.2 μm filtered
niveau d'endotoxine Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Plasmids, Primers & others Plasmids, Primers & others IL1R1 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation CD121a (IL1R1 Antibody Extrait)
Sujet The 35F5 antibody reacts with the type-I interleukin (IL-1) receptor (CD121a) expressed by T-cell, fibroblast, and epithelial cell lines such as EL-4 and 3T3. Expression of the 35F5 epitope on normal mouse thymocytes and splenocytes is undetectable by flow cytometry. CD121a is an 80-kDa type-I transmembrane glycoprotein, a member of the Ig superfamily which mediates the inflammatory responses of leukocytes to IL-1. mAb 35F5 is capable of blocking the binding of IL-1alpha, IL-1beta, and IL-1 receptor antagonist to the type-I IL-1 receptor. The 35F5 mAb is highly specific for the type-I IL-1 receptor and does not react with the type-II IL-1 receptor (CD121b) which is present on murine macrophage and B-cell lines and polymorphonuclear leukocytes (PMN) and PMN progenitors.
Synonyms: IL-1 Receptor, Type I/p80
Pathways Signalisation NF-kappaB, Carbohydrate Homeostasis, Cancer Immune Checkpoints
Indications d'application Flow cytometry: Since this antigen may be expressed at low density on the cell surface, investigators may wish to perform staining using Biotin Goat Anti-Rat Ig (Cat. No. 554014) coupled with a bright third-step reagent, such as PE Streptavidin (Cat. No. 554061).
Western Blot: The 35F5 antibody has been reported to be useful for western blotting of non-reduced proteins and being non-reactive to reduced proteins.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Agent conservateur Azide free
Stock 4 °C
Stockage commentaire Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Produit citée dans: McIntyre, Stepan, Kolinsky, Benjamin, Plocinski, Kaffka, Campen, Chizzonite, Kilian: "Inhibition of interleukin 1 (IL-1) binding and bioactivity in vitro and modulation of acute inflammation in vivo by IL-1 receptor antagonist and anti-IL-1 receptor monoclonal antibody." dans: The Journal of experimental medicine, Vol. 173, Issue 4, pp. 931-9, 1991 (PubMed).

Chizzonite, Truitt, Kilian, Stern, Nunes, Parker, Kaffka, Chua, Lugg, Gubler: "Two high-affinity interleukin 1 receptors represent separate gene products." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 20, pp. 8029-33, 1989 (PubMed).

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