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IL1R1 anticorps

IL1R1 Reactivité: Souris WB, IP, FACS, BR Hôte: Rat Monoclonal 35F5 unconjugated
N° du produit ABIN1176949
  • Antigène Voir toutes IL1R1 Anticorps
    IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))
    Reactivité
    • 120
    • 67
    • 53
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Hôte
    • 113
    • 14
    • 9
    • 5
    • 2
    • 1
    Rat
    Clonalité
    • 120
    • 24
    Monoclonal
    Conjugué
    • 61
    • 17
    • 11
    • 8
    • 8
    • 8
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Cet anticorp IL1R1 est non-conjugé
    Application
    • 123
    • 65
    • 46
    • 39
    • 15
    • 15
    • 13
    • 13
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Blocking Reagent (BR)
    Marque
    BD Pharmingen™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Stérilité
    0.2 μm filtered
    niveau d'endotoxine
    Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
    Immunogène
    IL-1 receptors from the C57BL/6 mouse T lymphoma EL4.IL2
    Clone
    35F5
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product IL1R1 Anticorps primaire
  • Indications d'application
    Flow cytometry: Since this antigen may be expressed at low density on the cell surface, investigators may wish to perform staining using Biotin Goat Anti-Rat Ig (Cat. No. 554014) coupled with a bright third-step reagent, such as PE Streptavidin (Cat. No. 554061).
    Western Blot: The 35F5 antibody has been reported to be useful for western blotting of non-reduced proteins and being non-reactive to reduced proteins.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
    Agent conservateur
    Azide free
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
  • McIntyre, Stepan, Kolinsky, Benjamin, Plocinski, Kaffka, Campen, Chizzonite, Kilian: "Inhibition of interleukin 1 (IL-1) binding and bioactivity in vitro and modulation of acute inflammation in vivo by IL-1 receptor antagonist and anti-IL-1 receptor monoclonal antibody." dans: The Journal of experimental medicine, Vol. 173, Issue 4, pp. 931-9, (1991) (PubMed).

    Chizzonite, Truitt, Kilian, Stern, Nunes, Parker, Kaffka, Chua, Lugg, Gubler: "Two high-affinity interleukin 1 receptors represent separate gene products." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 20, pp. 8029-33, (1989) (PubMed).

  • Antigène
    IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))
    Autre désignation
    CD121a (IL1R1 Produits)
    Synonymes
    anticorps CD121A, anticorps D2S1473, anticorps IL-1R-alpha, anticorps IL1R, anticorps IL1RA, anticorps P80, anticorps CD121a, anticorps CD121b, anticorps IL-iR, anticorps Il1r-1, anticorps DER4, anticorps Fit-1, anticorps Ly84, anticorps ST2L, anticorps St2, anticorps St2-rs1, anticorps T1, anticorps T1/ST2, anticorps interleukin 1 receptor type 1, anticorps interleukin 1 receptor, type I, anticorps interleukin 1 receptor-like 1, anticorps IL1R1, anticorps Il1r1, anticorps Il1rl1
    Sujet
    The 35F5 antibody reacts with the type-I interleukin (IL-1) receptor (CD121a) expressed by T-cell, fibroblast, and epithelial cell lines such as EL-4 and 3T3. Expression of the 35F5 epitope on normal mouse thymocytes and splenocytes is undetectable by flow cytometry. CD121a is an 80-kDa type-I transmembrane glycoprotein, a member of the Ig superfamily which mediates the inflammatory responses of leukocytes to IL-1. mAb 35F5 is capable of blocking the binding of IL-1alpha, IL-1beta, and IL-1 receptor antagonist to the type-I IL-1 receptor. The 35F5 mAb is highly specific for the type-I IL-1 receptor and does not react with the type-II IL-1 receptor (CD121b) which is present on murine macrophage and B-cell lines and polymorphonuclear leukocytes (PMN) and PMN progenitors.
    Synonyms: IL-1 Receptor, Type I/p80
    Pathways
    Signalisation NF-kappaB, Carbohydrate Homeostasis, Cancer Immune Checkpoints
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