IL-10 anticorps
-
- Antigène Voir toutes IL-10 (IL10) Anticorps
- IL-10 (IL10) (Interleukin 10 (IL10))
-
Reactivité
- Souris
-
Hôte
- Rat
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp IL-10 est non-conjugé
-
Application
- ELISA, Neutralization (Neut)
- Marque
- BD Pharmingen™
- Attributs du produit
-
Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-10. Neutralization activity may be measured with a proliferation assay using 5 ng/mL recombinant mouse IL-10 (Cat. No. 550070) and 0.1 ng/mL recombinant mouse IL-4 (Cat. No. 550067) to co-stimulate MC/9 cells at 1x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with the recombinant mouse IL-10 for 60 min).
50% Neutralization (ND50) at 2 - 8 ng/mL
>95% Neutralization 3 - 25 µg/mL - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Stérilité
- 0.2 μm filtered
- niveau d'endotoxine
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- Immunogène
- Mouse IL-10
- Clone
- JES5-2A5
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product IL10 Anticorps primaire
-
-
- Indications d'application
- ELISA: The purified format of the JES5-2A5 antibody (Cat. No. 551215) has been reported to be useful as a capture antibody in sandwich ELISA for mouse IL-10. Purified JES5-2A5 antibody can be paired with the biotinylated SXC-1 antibody (Cat. No. 554423) or biotinylated JES5-16E3 antibody (Cat. No. 554465) and recombinant mouse IL-10 (Cat. No. 550070) as the standard. Purified JES5-2A5 antibody should be titrated (suggested 2-6 µg/mL) to determine the optimal concentration for ELISA capture. An appropriate range of concentrations of the mouse IL-10 standard for obtaining a linear standard curve is 2 ng/mL to 15 pg/mL mouse IL-10.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- Agent conservateur
- Azide free
- Stock
- 4 °C
- Stockage commentaire
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
-
-
Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." dans: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
: "Induction of cytokines in mice with parainfluenza pneumonia." dans: Journal of virology, Vol. 69, Issue 2, pp. 1288-91, (1995) (PubMed).
: "Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1246-53, (1994) (PubMed).
: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." dans: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).
: "
-
Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." dans: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
-
- Antigène
- IL-10 (IL10) (Interleukin 10 (IL10))
- Autre désignation
- IL-10 (IL10 Produits)
- Synonymes
- anticorps CSIF, anticorps GVHDS, anticorps IL-10, anticorps IL10A, anticorps TGIF, anticorps Il-10, anticorps IL10X, anticorps interleukin 10, anticorps IL10, anticorps Il10
- Sujet
- The JES5-2A5 antibody reacts with mouse IL-10. The immunogen used to generate the JES5-2A5 hybridoma was E. coli-expressed mouse IL-10. This is a neutralizing antibody.
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
-