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eIF4EBP1 anticorps (pThr69) (Alexa Fluor 647)

EIF4EBP1 Reactivité: Humain ICS Hôte: Souris Monoclonal M34-273 Alexa Fluor 647
N° du produit ABIN1177025
  • Antigène Voir toutes eIF4EBP1 (EIF4EBP1) Anticorps
    eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
    Épitope
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    pThr69
    Reactivité
    • 242
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    • 21
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    • 7
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    • 2
    • 1
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    Humain
    Hôte
    • 256
    • 18
    • 4
    Souris
    Clonalité
    • 237
    • 43
    Monoclonal
    Conjugué
    • 116
    • 25
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    • 6
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    • 1
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    Cet anticorp eIF4EBP1 est conjugé à/à la Alexa Fluor 647
    Application
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    Intracellular Staining (ICS)
    Marque
    BD Phosflow™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Phosphorylated Human 4EBP1 (pT69) Peptide
    Clone
    M34-273
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product EIF4EBP1 Anticorps primaire
  • Indications d'application
    Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.
    Volume d'échantillon
    20 μL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Hay, Sonenberg: "Upstream and downstream of mTOR." dans: Genes & development, Vol. 18, Issue 16, pp. 1926-45, (2004) (PubMed).

    Gingras, Raught, Gygi, Niedzwiecka, Miron, Burley, Polakiewicz, Wyslouch-Cieszynska, Aebersold, Sonenberg: "Hierarchical phosphorylation of the translation inhibitor 4E-BP1." dans: Genes & development, Vol. 15, Issue 21, pp. 2852-64, (2001) (PubMed).

  • Antigène
    eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
    Autre désignation
    4EBP1 (EIF4EBP1 Produits)
    Synonymes
    anticorps 4E-BP1, anticorps 4EBP1, anticorps BP-1, anticorps PHAS-I, anticorps 4e-bp1, anticorps AA959816, anticorps 153432_at, anticorps 43-BP, anticorps 4E-BP, anticorps 4EBP, anticorps 4e-bp, anticorps 4eBP, anticorps 4ebp, anticorps BcDNA.HL08053, anticorps BcDNA:HL08053, anticorps CG8846, anticorps CT8705, anticorps Dm 4E-BP, anticorps Dmel\\CG8846, anticorps Phas1, anticorps Thor/EIF4EBP, anticorps anon-WO0172774.158, anticorps d4E-BP, anticorps d4E-BP1, anticorps d4EBP, anticorps d4e-bp, anticorps d4eBP, anticorps dthor, anticorps eIF4E-BP, anticorps l(2)06270, anticorps l(2)k07736, anticorps l(2)k13517, anticorps pp20, anticorps thor, anticorps EIF4EBP1, anticorps fc04h09, anticorps wu:fc04h09, anticorps zgc:64137, anticorps eukaryotic translation initiation factor 4E binding protein 1, anticorps CG8846 gene product from transcript CG8846-RA, anticorps EIF4EBP1, anticorps Eif4ebp1, anticorps Thor, anticorps eif4ebp1
    Sujet
    The eukaryotic translation initiation factor 4E-Binding Protein 1 (4EBP1) is a phosphorylated heat- and acid-stable protein (PHAS-I or PHAS-1), and it is regulated by insulin. It is a member of the eIF4E-Binding Protein Family, which also includes the proteins 4EBP2 and 4EBP3. 4EBP1 binds with eukaryotic translation Initiation Factor 4E (eIF4E), which prevents its assembly into the eIF4E complex and inhibits cap-dependent translation. When 4EBP1 is phosphorylated, this binding is disrupted, allowing cap-dependent translation to be activated. Phosphorylation of 4EBP1 is required for protein synthesis, and it mediates the regulation of protein translation by stimuli that signal through the phosphoinositide 3 (PI3) kinase pathway. We found that threonine 69 (T69) is phosphorylated in resting human peripheral blood monocytes, but it is almost undetectable in resting lymphocytes. PI3 kinase inhibitors, such as LY294002 down-regulate the phosphorylation level of 4EBP1 (pT69) in monocytes. The M34-273 monoclonal antibody recognizes the phosphorylated T69 of activated human 4EBP1.
    Synonyms: 4E-BP1. EIF4EBP1, P/OKCL.6, PHAS-I, PHAS-1
    Pathways
    Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Regulation of Cell Size, BCR Signaling
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