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AKT1 anticorps (pSer473) (Alexa Fluor 647)

AKT1 Reactivité: Humain, Souris ICS Hôte: Souris Monoclonal M89-61 Alexa Fluor 647
N° du produit ABIN1177030
  • Antigène Voir toutes AKT1 Anticorps
    AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
    Épitope
    • 97
    • 48
    • 37
    • 34
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    • 22
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    • 17
    • 16
    • 11
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    • 2
    • 2
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    • 2
    • 2
    • 2
    pSer473
    Reactivité
    • 561
    • 366
    • 288
    • 55
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    • 34
    • 20
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    • 12
    • 4
    • 4
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain, Souris
    Hôte
    • 481
    • 100
    • 7
    • 2
    • 1
    Souris
    Clonalité
    • 433
    • 157
    Monoclonal
    Conjugué
    • 319
    • 39
    • 28
    • 25
    • 25
    • 19
    • 16
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    • 12
    • 4
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Cet anticorp AKT1 est conjugé à/à la Alexa Fluor 647
    Application
    • 448
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    • 193
    • 87
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    • 52
    • 31
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    • 15
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    • 9
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    • 4
    • 3
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Intracellular Staining (ICS)
    Marque
    BD Phosflow™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Phosphorylated Human Akt1 (pS473) Peptide
    Clone
    M89-61
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product AKT1 Anticorps primaire
  • Volume d'échantillon
    20 μL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
  • Prinz, Mendler, Masouris, Durner, Oberneder, Noessner: "High DGK-α and disabled MAPK pathways cause dysfunction of human tumor-infiltrating CD8+ T cells that is reversible by pharmacologic intervention." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 12, pp. 5990-6000, (2012) (PubMed).

    Schlickeiser, Stanojlovic, Appelt, Vogt, Vogel, Haase, Ritter, Volk, Pleyer, Sawitzki: "Control of TNF-induced dendritic cell maturation by hybrid-type N-glycans." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 9, pp. 5201-11, (2011) (PubMed).

    Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, (1999) (PubMed).

    Ferrigno, Silver: "Regulated nuclear localization of stress-responsive factors: how the nuclear trafficking of protein kinases and transcription factors contributes to cell survival." dans: Oncogene, Vol. 18, Issue 45, pp. 6129-34, (1999) (PubMed).

    Kandel, Hay: "The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB." dans: Experimental cell research, Vol. 253, Issue 1, pp. 210-29, (1999) (PubMed).

    Alessi, Andjelkovic, Caudwell, Cron, Morrice, Cohen, Hemmings: "Mechanism of activation of protein kinase B by insulin and IGF-1." dans: The EMBO journal, Vol. 15, Issue 23, pp. 6541-51, (1997) (PubMed).

    Datta, Dudek, Tao, Masters, Fu, Gotoh, Greenberg: "Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery." dans: Cell, Vol. 91, Issue 2, pp. 231-41, (1997) (PubMed).

  • Antigène
    AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
    Autre désignation
    Akt (AKT1 Produits)
    Synonymes
    anticorps AKT, anticorps CWS6, anticorps PKB, anticorps PKB-ALPHA, anticorps PRKBA, anticorps RAC, anticorps RAC-ALPHA, anticorps Akt, anticorps Rac, anticorps PKB/Akt, anticorps PKBalpha, anticorps AKT/PKB, anticorps AKT1, anticorps Akt/PKB, anticorps Akt1, anticorps CG4006, anticorps D-Akt, anticorps DAKT1, anticorps DAKT1/PKB, anticorps DAkt, anticorps DAkt1, anticorps DPKB, anticorps DRAC-PK, anticorps DRAC-PK66, anticorps DRAC-PK85, anticorps Dakt, anticorps Dakt1, anticorps Dmel\\CG4006, anticorps Dpkb, anticorps PKB/AKT, anticorps PKB/dAKT, anticorps RacPK, anticorps akt, anticorps akt1, anticorps dAKT, anticorps dAKT/dPKB, anticorps dAKT1, anticorps dAkt, anticorps dAkt/PKB, anticorps dAkt1, anticorps dPKB, anticorps dakt, anticorps dakt1, anticorps l(3)04226, anticorps l(3)89Bq, anticorps p-Akt, anticorps pAkt, anticorps ACT-5, anticorps akt-1, anticorps pkb, anticorps v-akt, anticorps v-akt1, anticorps xAct, anticorps ATAKT1, anticorps F18A8.2, anticorps F18A8_2, anticorps K+ transporter 1, anticorps POTASSIUM TRANSPORTER, anticorps AKT serine/threonine kinase 1, anticorps thymoma viral proto-oncogene 1, anticorps CG4006 gene product from transcript CG4006-RE, anticorps actin beta, anticorps v-akt murine thymoma viral oncogene homolog 1 S homeolog, anticorps v-akt murine thymoma viral oncogene homolog 1, anticorps K+ transporter 1, anticorps Serine/threonine-protein kinase akt-1, anticorps AKT1, anticorps Akt1, anticorps ACTB, anticorps akt1.S, anticorps akt1, anticorps KT1, anticorps akt-1
    Sujet
    Akt [also known as PKB (Protein kinase B) or RAC-PK (Related to the A and C kinases)] is a family of serine/threonine kinases that contains a pleckstrin homology (PH) domain. PH domains play important roles in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (alpha), Akt2 (beta) and Akt3 (gamma)], they are thought to be regulated similarly. Akt is activated by insulin and growth factors by a mechanism involving phosphoinositide 3-OH kinase. Phosphoinositide 3-OH kinases products bind to the PH domain, resulting in translocation of Akt to the plasma membrane and activation of Akt to phospho-Akt by upstream kinases. Akt is phosphorylated within the activation loop at threonine 308 and the C-terminus at serine 473 (S473). Phospho-Akt promotes cell survival by inhibiting apoptosis. Specifically, phospho-Akt1 has been shown to phosphorylate Bad, a member of the Bcl-2 family that promotes cell death. This phosphorylation results in the inactivation of the proapoptotic function of Bad. The Akt molecule is thus considered to link extracellular survival signals (growth factors) with the apoptotic machinery (BAD). Akt is also a key mediator of the metabolic effects of insulin. Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors. The M89-61 antibody recognizes Akt phosphorylated at S473. This phosphorylation site is shared by all three isoforms of Akt. The homologous phosphorylation sites in Akt2 and Akt3 are S474 and S472, respectively.
    Synonyms: Akt1, Akt2, Akt3, PKBalpha, PKBbeta, PKBgamma, RAC-PKalpha, RAC-PKbeta, RAC-PKgamma, STK-2
    Pathways
    Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg
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