IKBKG anticorps (pSer376) (PE)

N° du produit ABIN1177069

Détail du produit anti-IKBKG anticorps (PE)

Antigène: IKBKG (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))

Épitope: pSer376

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IKBKG est conjugé à/à la PE

Application: Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human IKKgamma Peptide

Clone: N19-39

Isotype: IgG1 kappa

Information d'application

Volume d'échantillon: 5 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Références pour anticorps anti-IKBKG (PE) (ABIN1177069)

Shifera: "The zinc finger domain of IKKγ (NEMO) protein in health and disease." dans: Journal of cellular and molecular medicine, Vol. 14, Issue 10, pp. 2404-14, (2010) (PubMed).

Carter, Pennington, Ungurait, Ballard: "In vivo identification of inducible phosphoacceptors in the IKKgamma/NEMO subunit of human IkappaB kinase." dans: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 19642-8, (2003) (PubMed).

Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, (1998) (PubMed).

Rothwarf, Zandi, Natoli, Karin: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." dans: Nature, Vol. 395, Issue 6699, pp. 297-300, (1998) (PubMed).

DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." dans: Nature, Vol. 388, Issue 6642, pp. 548-54, (1997) (PubMed).

Mercurio, Zhu, Murray, Shevchenko, Bennett, Li, Young, Barbosa, Mann, Manning, Rao: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." dans: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, (1997) (PubMed).

Détails sur IKBKG

Antigène: IKBKG (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))

Abstract: IKBKG Produits

Synonymes: anticorps AMCBX1, anticorps FIP-3, anticorps FIP3, anticorps Fip3p, anticorps IKK-gamma, anticorps IP, anticorps IP1, anticorps IP2, anticorps IPD2, anticorps NEMO, anticorps ZC2HC9, anticorps 1110037D23Rik, anticorps AI848108, anticorps AI851264, anticorps AW124339, anticorps IKK[g], anticorps Nemo, anticorps nemo, anticorps fj33c07, anticorps wu:fj33c07, anticorps zgc:113492, anticorps MGC97885, anticorps IKBKG, anticorps IKBKG_1, anticorps IKBKG_2, anticorps CG16910, anticorps DmIKK-gamma, anticorps DmIKKgamma, anticorps Dmel\\CG16910, anticorps Dmikkgamma, anticorps IKK, anticorps IKK[[gamma]], anticorps IKKg, anticorps IKKgamma, anticorps KEY, anticorps Kenny, anticorps Key, anticorps dIKK, anticorps dIKK-gamma, anticorps dmIKKgamma, anticorps inhibitor of nuclear factor kappa B kinase subunit gamma, anticorps inhibitor of kappaB kinase gamma, anticorps inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma, anticorps inhibitor of nuclear factor kappa B kinase subunit gamma L homeolog, anticorps kenny, anticorps IKBKG, anticorps Ikbkg, anticorps ikbkg, anticorps ikbkg.L, anticorps IKBKG_1, anticorps key

Sujet: The N19-39 monoclonal antibody specifically binds to the IKK-gamma protein phosphorylated at the Ser376 site, ie, IKKgamma (pS376). IKKgamma is also known an NEMO (NF-kappa-B essential modifier) and is encoded by the IKBKG (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma) gene. The nuclear transcription factor kappa-B, NF-kappaB, is controlled by interaction with an inhibitory subunit, IkappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, IkappaB becomes degraded and NF-kappaB is released to the nucleus. The release of IkappaB from NF-kappaB is thought to be a critical point in the activation of NF-kappaB signal pathways. Activated NF-kappaB regulates genes involved in various pathways including inflammation, immunity, and cell survival signaling pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKalpha and IKKbeta (also called IKK-1 and IKK-2), which phosphorylate critical residues of IkappaB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKalpha, IKKbeta as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKgamma. IKKalpha and IKKbeta become activated following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK)4 and MEKK1. IKKbeta has been shown to phosphorylate IKKgamma at serine 376 in response to signaling through the TNF receptor or the Tax oncoprotein of human T-cell leukemia virus type 1. IKKgamma has an essential regulatory role in the IKK complex and is required for the activation of the NF-kappaB pathway in response to multiple cellular stimuli. IKKgamma isoforms migrate as a doublet of ~50-55 kDa upon Western blot analysis.
Synonyms: IKBKG, IKKgamma, IKKG, IKK-gamma, AMCBX1, FIP3, IKKAP1, IP1, IP2, IPD2, NEMO

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, M Phase, Production of Molecular Mediator of Immune Response, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins