LAT anticorps (pTyr171) (Alexa Fluor 488)

N° du produit ABIN1177078

Détail du produit anti-LAT anticorps (Alexa Fluor 488)

Antigène: LAT (Linker For Activation of T Cells (LAT))

Épitope: pTyr171

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LAT est conjugé à/à la Alexa Fluor 488

Application: Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography

Immunogène: Phosphorylated Human LAT

Clone: I58-1169

Isotype: IgG1 kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-LAT (Alexa Fluor 488) (ABIN1177078)

Cho, Velikovsky, Swaminathan, Houtman, Samelson, Mariuzza: "Structural basis for differential recognition of tyrosine-phosphorylated sites in the linker for activation of T cells (LAT) by the adaptor Gads." dans: The EMBO journal, Vol. 23, Issue 7, pp. 1441-51, (2004) (PubMed).

Janssen, Zhang: "Adaptor proteins in lymphocyte activation." dans: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, (2003) (PubMed).

Samelson: "Signal transduction mediated by the T cell antigen receptor: the role of adapter proteins." dans: Annual review of immunology, Vol. 20, pp. 371-94, (2002) (PubMed).

Zhu, Janssen, Zhang: "Minimal requirement of tyrosine residues of linker for activation of T cells in TCR signaling and thymocyte development." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 325-33, (2002) (PubMed).

Lin, Weiss: "Identification of the minimal tyrosine residues required for linker for activation of T cell function." dans: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 29588-95, (2001) (PubMed).

Paz, Wang, Clarke, Lu, Stokoe, Abo: "Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells." dans: The Biochemical journal, Vol. 356, Issue Pt 2, pp. 461-71, (2001) (PubMed).

Détails sur LAT

Antigène: LAT (Linker For Activation of T Cells (LAT))

Autre désignation: LAT (LAT Produits)

Synonymes: anticorps LAT, anticorps lat, anticorps LAT1, anticorps pp36, anticorps linker for activation of T-cells, anticorps linker for activation of T cells, anticorps LAT, anticorps lat, anticorps Lat

Sujet: Engagement of the T cell receptor (TCR) induces signal transduction pathways that enhance gene transcription and cellular proliferation and differentiation. TCR ligation results in the recruitment and activation of multiple protein tyrosine kinases (PTKs), including lck, fyn, and ZAP70. Adaptor proteins, such as Grb2 and SLP-76, relay the signal to downstream effector molecules. LAT (linker for activation of T cells) is a substrate of the activated ZAP70 and functions to bridge the activated TCR and its associated PTKs with tyrosine kinase substrates. LAT is expressed as 36- and 38-kDa forms that result from post-translational modification, and as a 42-kDa form that results from alternative splicing. LAT is an integral membrane protein that is phosphorylated at five tyrosine sites upon TCR ligation. Following phosphorylation, LAT binds a number of important signaling molecules, including Grb2, Vav, PLCgamma1, and the p85 subunit of PI3K. Multiple studies have shown that functional LAT is required for T lymphocyte activation and thymocyte development. The I58-1169 monoclonal antibody recognizes the phosphorylated tyrosine 171 (pY171) of LAT, which is one of the phosphotyrosine sites required for binding phosphoinositide 3-kinase, Grb2, and Gads.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway