MAPKAP Kinase 2 anticorps (pThr334) (Alexa Fluor 647)

N° du produit ABIN1177088

Détail du produit anti-MAPKAP Kinase 2 anticorps (Alexa Fluor 647)

Antigène: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Épitope: pThr334

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAPKAP Kinase 2 est conjugé à/à la Alexa Fluor 647

Application: Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human MAPKAPK-2 Peptide

Clone: P24-694

Isotype: IgG1 kappa

Information d'application

Volume d'échantillon: 5 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Références pour anticorps anti-MAPKAP Kinase 2 (Alexa Fluor 647) (ABIN1177088)

Manke, Nguyen, Lim, Stewart, Elia, Yaffe: "MAPKAP kinase-2 is a cell cycle checkpoint kinase that regulates the G2/M transition and S phase progression in response to UV irradiation." dans: Molecular cell, Vol. 17, Issue 1, pp. 37-48, (2005) (PubMed).

Wang, Xu, Wang, Young, Gaestel, Feuerstein: "Mitogen-activated protein kinase-activated protein (MAPKAP) kinase 2 deficiency protects brain from ischemic injury in mice." dans: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 43968-72, (2002) (PubMed).

Heidenreich, Neininger, Schratt, Zinck, Cahill, Engel, Kotlyarov, Kraft, Kostka, Gaestel, Nordheim: "MAPKAP kinase 2 phosphorylates serum response factor in vitro and in vivo." dans: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14434-43, (1999) (PubMed).

Engel, Kotlyarov, Gaestel: "Leptomycin B-sensitive nuclear export of MAPKAP kinase 2 is regulated by phosphorylation." dans: The EMBO journal, Vol. 17, Issue 12, pp. 3363-71, (1998) (PubMed).

Krump, Sanghera, Pelech, Furuya, Grinstein: "Chemotactic peptide N-formyl-met-leu-phe activation of p38 mitogen-activated protein kinase (MAPK) and MAPK-activated protein kinase-2 in human neutrophils." dans: The Journal of biological chemistry, Vol. 272, Issue 2, pp. 937-44, (1997) (PubMed).

Clifton, Young, Cohen: "A comparison of the substrate specificity of MAPKAP kinase-2 and MAPKAP kinase-3 and their activation by cytokines and cellular stress." dans: FEBS letters, Vol. 392, Issue 3, pp. 209-14, (1996) (PubMed).

Rouse, Cohen, Trigon, Morange, Alonso-Llamazares, Zamanillo, Hunt, Nebreda: "A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins." dans: Cell, Vol. 78, Issue 6, pp. 1027-37, (1994) (PubMed).

Détails sur MAPKAP Kinase 2

Antigène: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Autre désignation: MAPKAPK-2 (MAPKAPK2 Produits)

Synonymes: anticorps mapkapk2, anticorps wu:fb30e10, anticorps wu:fc56c10, anticorps wu:fi03a01, anticorps MGC78852, anticorps MAPKAPK2, anticorps AA960234, anticorps MAPKAP-K2, anticorps MK-2, anticorps MK2, anticorps Rps6kc1, anticorps mitogen-activated protein kinase-activated protein kinase 2a, anticorps mitogen-activated protein kinase-activated protein kinase 2 L homeolog, anticorps mitogen-activated protein kinase-activated protein kinase 2, anticorps MAP kinase-activated protein kinase 2, anticorps mapkapk2a, anticorps mapkapk2.L, anticorps MAPKAPK2, anticorps Mapkapk2

Sujet: The P24-694 monoclonal antibody specifically binds to the phosphorylated T334 site (pT334) of MAPKAPK-2. MAPKAPK-2 is a serine/threonine protein kinase. This ~49 kDa member of the MAPKAPK family of protein kinases is also known as mitogen-activated protein kinase-activated protein kinase 2. MAPKAPK-2 is phosphorylated and activated by p38 MAP kinase in response to stress, cytokines and chemokines. MAPKAPK-2 is phosphorylated on multiple sites including Thr222, Ser272 and Thr334. Phosphorylation of any two of these three amino acid residues seems to be required for the activation of this kinase that serves multiple cellular functions. Phosphorylation of Thr334 was reported to be essential for nuclear export of the heterodimer formed between p38 MAPK and MAPKAPK-2. Mice deficient in MAPKAPK-2 have been shown to be protected from ischemic injury. MAPKAPK-2 is also reported to serve as a cell cycle checkpoint kinase in response to UV irradiation. The heat shock protein, HSP27 was shown to be one of the major substrates of MAPK and MAPKAPK-2.
Synonyms: MAP kinase-activated protein kinase 2, MAPKAP kinase 2, MK2, MAPK2

Pathways: Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades