PDGFRB anticorps (pTyr857) (Alexa Fluor 488)

N° du produit ABIN1177131

Détail du produit anti-PDGFRB anticorps (Alexa Fluor 488)

Antigène: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Épitope: pTyr857

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDGFRB est conjugé à/à la Alexa Fluor 488

Application: Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human PDGFRbeta Peptide

Clone: J24-618

Isotype: IgG1 kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Références pour anticorps anti-PDGFRB (Alexa Fluor 488) (ABIN1177131)

Liu, Oh, Horner, Rogers, Schnitzer: "Organized endothelial cell surface signal transduction in caveolae distinct from glycosylphosphatidylinositol-anchored protein microdomains." dans: The Journal of biological chemistry, Vol. 272, Issue 11, pp. 7211-22, (1997) (PubMed).

Claesson-Welsh: "Platelet-derived growth factor receptor signals." dans: The Journal of biological chemistry, Vol. 269, Issue 51, pp. 32023-6, (1995) (PubMed).

Détails sur PDGFRB

Antigène: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Autre désignation: CD140b (PDGFRB Produits)

Synonymes: anticorps AI528809, anticorps CD140b, anticorps PDGFR-1, anticorps Pdgfr, anticorps CD140B, anticorps IBGC4, anticorps JTK12, anticorps PDGFR, anticorps PDGFR1, anticorps platelet derived growth factor receptor, beta polypeptide, anticorps platelet derived growth factor receptor beta, anticorps Pdgfrb, anticorps PDGFRB

Sujet: Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase. PDGFR is composed of PDGFRalpha (CD140a) and/or PDGFRbeta (CD140b) polypeptides. Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to alphaalpha PDGFR, PDGF-AB binds to both alphaalpha and alphabeta PDGFR, and PDGF-BB binds to all three PDGFRs. Ligand binding induces dimerization and activation of the receptor. Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated. PDGFR localizes primarily to membrane invaginations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-gamma. The J24-618 monoclonal antibody recognizes the phosphorylated tyrosine 857 (pY857) in the tyrosine kinase domain 2 of CD140b, which is required for maximal receptor kinase activity. The orthologous phosphorylation site in mouse PDGFRbeta is Y856.

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling