STAT1 anticorps (pTyr701) (Alexa Fluor 647)

N° du produit ABIN1177190

Détail du produit anti-STAT1 anticorps (Alexa Fluor 647)

Antigène: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Épitope: pTyr701

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp STAT1 est conjugé à/à la Alexa Fluor 647

Application: Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human Stat1 Peptide

Clone: 4a

Isotype: IgG2a

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Références pour anticorps anti-STAT1 (Alexa Fluor 647) (ABIN1177190)

Tanaka, Saito, Kunisawa, Kurashima, Wake, Suzuki, Shultz, Kiyono, Ishikawa: "Development of mature and functional human myeloid subsets in hematopoietic stem cell-engrafted NOD/SCID/IL2rγKO mice." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 12, pp. 6145-55, (2012) (PubMed).

Suni, Maino: "Flow cytometric analysis of cell signaling proteins." dans: Methods in molecular biology (Clifton, N.J.), Vol. 717, pp. 155-69, (2011) (PubMed).

Perez, Mitchell, Campos, Gao, Li, Nolan: "Multiparameter analysis of intracellular phosphoepitopes in immunophenotyped cell populations by flow cytometry." dans: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." dans: Oncogene, Vol. 19, Issue 21, pp. 2468-73, (2000) (PubMed).

Darnell: "STATs and gene regulation." dans: Science (New York, N.Y.), Vol. 277, Issue 5332, pp. 1630-5, (1997) (PubMed).

Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." dans: Cell, Vol. 74, Issue 6, pp. 1135-45, (1993) (PubMed).

Détails sur STAT1

Antigène: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Autre désignation: Stat1 (STAT1 Produits)

Synonymes: anticorps CANDF7, anticorps ISGF-3, anticorps STAT91, anticorps 2010005J02Rik, anticorps AA408197, anticorps XStat1, anticorps STAT4, anticorps DD6G4-4, anticorps STAT, anticorps STAT1, anticorps dZ182H3.3, anticorps si:by134g18.2, anticorps si:by51f19.1, anticorps si:dz182h3.3, anticorps si:dz199m19.1, anticorps si:dz73o4.2, anticorps stat1, anticorps signal transducer and activator of transcription 1, anticorps signal transducer and activator of transcription 1 L homeolog, anticorps signal transducer and activator of transcription 1, 91kDa, anticorps signal transducer and activator of transcription 4, anticorps signal transducer and activator of transcription 1a, anticorps STAT1, anticorps Stat1, anticorps stat1.L, anticorps stat4, anticorps stat1a

Sujet: Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84-kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs). The 4a monoclonal antibody recognizes the phosphorylated Y701 in Stat1alpha and Stat1beta.

Pathways: Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events