STAT1 anticorps (Signal Transducer and Activator of Transcription 1, 91kDa) (pTyr701) (Alexa Fluor 647)

Details for Product anti-STAT1 Antibody No. ABIN1177190
Antigène
  • CANDF7
  • ISGF-3
  • STAT91
  • 2010005J02Rik
  • AA408197
  • XStat1
  • STAT4
  • DD6G4-4
  • STAT
  • STAT1
  • dZ182H3.3
  • si:by134g18.2
  • si:by51f19.1
  • si:dz182h3.3
  • si:dz199m19.1
  • si:dz73o4.2
  • stat1
  • signal transducer and activator of transcription 1
  • signal transducer and activator of transcription 1 L homeolog
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription 4
  • signal transducer and activator of transcription 1a
  • STAT1
  • Stat1
  • stat1.L
  • stat4
  • stat1a
Épitope
pTyr701
101
74
52
17
17
15
9
8
8
8
8
7
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris
564
314
282
33
29
26
22
22
16
13
11
7
7
5
2
2
2
1
1
1
1
1
Hôte
Souris
485
105
2
2
1
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp STAT1 est conjugé à/à la Alexa Fluor 647
18
14
13
11
11
11
11
11
11
6
6
5
5
5
5
5
4
2
2
2
Application
Intracellular Staining (ICS)
448
205
163
113
91
65
48
19
18
9
7
6
5
3
1
1
Options
Marque BD Phosflow™
Immunogène Phosphorylated Human Stat1 Peptide
Clone 4a
Isotype IgG2a
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmids, Primers & others STAT1 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Stat1 (STAT1 Antibody Extrait)
Sujet Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84-kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs). The 4a monoclonal antibody recognizes the phosphorylated Y701 in Stat1alpha and Stat1beta.
Pathways Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events
Volume d'échantillon 20 μL
Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Stockage commentaire Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Produit citée dans: Tanaka, Saito, Kunisawa, Kurashima, Wake, Suzuki, Shultz, Kiyono, Ishikawa: "Development of mature and functional human myeloid subsets in hematopoietic stem cell-engrafted NOD/SCID/IL2rγKO mice." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 12, pp. 6145-55, 2012 (PubMed).

Suni, Maino: "Flow cytometric analysis of cell signaling proteins." dans: Methods in molecular biology (Clifton, N.J.), Vol. 717, pp. 155-69, 2011 (PubMed).

Perez, Mitchell, Campos, Gao, Li, Nolan: "Multiparameter analysis of intracellular phosphoepitopes in immunophenotyped cell populations by flow cytometry." dans: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.], Vol. Chapter 6, pp. Unit 6.20, 2008 (PubMed).

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." dans: Oncogene, Vol. 19, Issue 21, pp. 2468-73, 2000 (PubMed).

Darnell: "STATs and gene regulation." dans: Science (New York, N.Y.), Vol. 277, Issue 5332, pp. 1630-5, 1997 (PubMed).

Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." dans: Cell, Vol. 74, Issue 6, pp. 1135-45, 1993 (PubMed).

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