Bcl-2 anticorps (pSer70) (Alexa Fluor 647)
-
- Antigène Voir toutes Bcl-2 (BCL2) Anticorps
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
-
Épitope
- pSer70
-
Reactivité
- Humain
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp Bcl-2 est conjugé à/à la Alexa Fluor 647
-
Application
- Intracellular Staining (ICS)
- Marque
- BD Phosflow™
- Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- Phosphorylated Peptide
- Clone
- N46-467
- Isotype
- IgG1
- Top Product
- Discover our top product BCL2 Anticorps primaire
-
-
- Volume d'échantillon
- 5 μL
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
-
-
Allyl isothiocyanate arrests cancer cells in mitosis, and mitotic arrest in turn leads to apoptosis via Bcl-2 protein phosphorylation." dans: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32259-67, (2011) (PubMed).
: "Complex inhibitory effects of nitric oxide on autophagy." dans: Molecular cell, Vol. 43, Issue 1, pp. 19-32, (2011) (PubMed).
: "Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy." dans: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2719-28, (2009) (PubMed).
: "Novel role for JNK as a stress-activated Bcl2 kinase." dans: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23681-8, (2001) (PubMed).
: "Phosphorylation of Bcl-2 is a marker of M phase events and not a determinant of apoptosis." dans: The Journal of biological chemistry, Vol. 273, Issue 30, pp. 18984-91, (1998) (PubMed).
: "
-
Allyl isothiocyanate arrests cancer cells in mitosis, and mitotic arrest in turn leads to apoptosis via Bcl-2 protein phosphorylation." dans: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32259-67, (2011) (PubMed).
-
- Antigène
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
- Autre désignation
- Bcl-2 (BCL2 Produits)
- Synonymes
- anticorps Bcl-2, anticorps PPP1R50, anticorps bcl-2, anticorps AW986256, anticorps C430015F12Rik, anticorps D630044D05Rik, anticorps D830018M01Rik, anticorps BCL-2, anticorps Bcl2, anticorps BCL2, apoptosis regulator, anticorps B cell leukemia/lymphoma 2, anticorps B-cell CLL/lymphoma 2a, anticorps B-cell CLL/lymphoma 2 protein, anticorps BCL2, anticorps Bcl2, anticorps bcl2a, anticorps BCL-2
- Sujet
-
The N46-467 monoclonal antibody specifically binds to Bcl-2 (pS70), ie, the Bcl-2 protein phosphorylated at the Ser70 site. Bcl-2 is a ~ 26 kDa intracellular, integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane. Bcl-2 is encoded by the BCL2 (B-cell CLL/lymphoma 2) gene and is also known as Apoptosis regulator Bcl-2. Members of the Bcl-2 family play a major role in regulating the response of cells to apoptotic signals. Bcl-2 is one of the anti-apoptotic members of the Bcl-2 family. Bcl-2 knockout mice showed pronounced lymphoid apoptosis and other apoptosis related lesions later in life. Bcl-2 is a proto-oncogene because it blocks apoptosis and provides a selective survival advantage in many cell types and thus contributes to tumorigenesis. It has been implicated in several types of cancers, such as breast, prostate, and melanoma . Bcl-2 contains multiple phosphorylation sites including Thr56, Ser70, Thr74 and Ser87. Phosphorylation of Bcl-2 Ser70 has been shown to be a mitotic marker. Phosphorylation at this site regulates Bcl-2's anti-apoptotic activity and has recently been implicated in promoting autophagy. Several studies have shown that Bcl-2 phosphorylation is caused by c-Jun N-terminal kinase (JNK).
Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, PPP1R50 - Pathways
- Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
-