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STAT5A anticorps (pTyr694) (Alexa Fluor 647)

STAT5A Reactivité: Humain, Souris, Rat, Mouton ICS Hôte: Souris Monoclonal 47-Stat5 Alexa Fluor 647
N° du produit ABIN1177249
  • Antigène Voir toutes STAT5A Anticorps
    STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
    Épitope
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    pTyr694
    Reactivité
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    Humain, Souris, Rat, Mouton
    Hôte
    • 178
    • 29
    • 2
    Souris
    Clonalité
    • 158
    • 51
    Monoclonal
    Conjugué
    • 119
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    Cet anticorp STAT5A est conjugé à/à la Alexa Fluor 647
    Application
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    Intracellular Staining (ICS)
    Marque
    BD Phosflow™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Phosphorylated Sheep Stat5 Peptide
    Clone
    47-Stat5
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product STAT5A Anticorps primaire
  • Indications d'application
    This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD Phosflow™ Fix Buffer I).
    Volume d'échantillon
    5 μL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
  • Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." dans: Oncogene, Vol. 19, Issue 21, pp. 2468-73, (2000) (PubMed).

    Imada, Leonard: "The Jak-STAT pathway." dans: Molecular immunology, Vol. 37, Issue 1-2, pp. 1-11, (2000) (PubMed).

    Liu, Gaffen, Goldsmith: "JAK/STAT signaling by cytokine receptors." dans: Current opinion in immunology, Vol. 10, Issue 3, pp. 271-8, (1998) (PubMed).

    Gouilleux, Wakao, Mundt, Groner: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." dans: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, (1994) (PubMed).

  • Antigène
    STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
    Autre désignation
    Stat5 (STAT5A Produits)
    Synonymes
    anticorps MGF, anticorps STAT5, anticorps AA959963, anticorps Stat5, anticorps STAT5A/MGF, anticorps STATA5, anticorps STAT5A, anticorps stat5, anticorps stat5b, anticorps signal transducer and activator of transcription 5A, anticorps signal transducer and activator of transcription 5a, anticorps STAT5A, anticorps Stat5a, anticorps stat5a, anticorps LOC100348518, anticorps LOC100716811
    Sujet
    Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat5 has been characterized and shown to be encoded by two separate genes, Stat5a and Stat5b that share over 90% identity at the amino acid level. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Both Stat5a and Stat5b share similarities, both are involved in IL-2 induced peripheral T cell proliferation. The peptide hormone, prolactin, binds to the prolactin receptor (PRLR) to initiate the lactogenic response. There are at least three forms of PRLR, however, only the long form is able to activate the 92-kDa Stat5 protein by inducing phosphorylation at Y694. Once phosphorylated, Stat5 becomes an essential transcription factor which binds to the beta-casein gene promoter. The presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2. The 47 monoclonal antibody recognizes the phosphorylated Y694 of Stat5a. The homologous phosphorylation site in Stat5b is Y699.
    Synonyms: Mammary gland factor, MGF, MPF
    Pathways
    Signalistation JAK/STAT, Signalisation RTK, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
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