IL-10RA anticorps

N° du produit ABIN1177268

Détail du produit anti-IL-10RA anticorps

Antigène: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-10RA est non-conjugé

Application: Flow Cytometry (FACS), Neutralization (Neut)

Marque: BD Pharmingen™

Attributs du produit: Neutralization Activity: This antibody has been reported to be useful for the neutralization of the activity elicited by recombinant mouse IL-10. Neutralization activity may be measured with a proliferation assay with 1x10^5 cells/mL D36 indicator cells using 6 ng/mL recombinant mouse IL-10 (Cat. No. 550070) costimulated with 50 pg/mL recombinant mouse IL-4 (Cat. No. 550067) (i.e preincubatiion of the antibody with the cells for 60 min)
50% Neutralization (ND50) at 0.1 - 1 ng/mL
>95% Neutralization at ≥ 1 - 15 ng/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogène: Purified recombinant ligand-binding domain of mIL-10R

Clone: 1B1-3a

Isotype: IgG1 kappa

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-IL-10RA (ABIN1177268)

OFarrell, Liu, Moore, Mui: "IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways." dans: The EMBO journal, Vol. 17, Issue 4, pp. 1006-18, (1998) (PubMed).

Schlaak, Schmitt, Hüls, Meyer zum Büschenfelde, Fleischer: "A sensitive and specific bioassay for the detection of human interleukin-10." dans: Journal of immunological methods, Vol. 168, Issue 1, pp. 49-54, (1994) (PubMed).

Détails sur IL-10RA

Antigène: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Autre désignation: CD210 (IL10RA Produits)

Synonymes: anticorps IL10R1, anticorps IL10RA, anticorps CD210, anticorps CD210a, anticorps CDW210A, anticorps HIL-10R, anticorps IL-10R1, anticorps IL10R, anticorps AW553859, anticorps CDw210, anticorps CDw210a, anticorps Il10r, anticorps mIL-10R, anticorps interleukin 10 receptor subunit alpha, anticorps interleukin 10 receptor, alpha, anticorps IL10RA, anticorps Il10ra

Sujet: The 1B1.3a antibody reacts with the extracellular region of mouse CD210 which is also known as the mouse IL-10 receptor (mIL-10R), it does not recognize the human IL-10R. The IL-10R is expressed by a variety of mouse cell types and cell lines including thymocytes, T cells, B cells, and monocytes. 1B1.3a is a neutralizing antibody and reportedly blocks the binding of human IL-10, which cross-reacts with the mouse IL-10R. The immunogen used for the generation of the 1B1.3a hybridoma was purified recombinant ligand-binding domain of mIL-10R.
Synonyms: IL-10 Receptor

Pathways: Growth Factor Binding