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NEDD9 anticorps (AA 82-398, AA 843)

NEDD9 Reactivité: Humain WB, IP Hôte: Souris Monoclonal 2G9 unconjugated
N° du produit ABIN129518
  • Antigène Voir toutes NEDD9 Anticorps
    NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))
    Épitope
    • 9
    • 9
    • 7
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 82-398, AA 843
    Reactivité
    • 46
    • 12
    • 12
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 39
    • 6
    • 1
    Souris
    Clonalité
    • 41
    • 5
    Monoclonal
    Conjugué
    • 24
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Cet anticorp NEDD9 est non-conjugé
    Application
    • 26
    • 18
    • 14
    • 8
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP)
    Attributs du produit
    Concentration Definition: by UV absorbance at 280 nm
    Immunogène
    Anti-HEF1 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 82-398 of human HEF1 protein (hHEF1, , predicted MW 92.8 kDa).
    Clone
    2G9
    Isotype
    IgG
    Top Product
    Discover our top product NEDD9 Anticorps primaire
  • Indications d'application
    This monoclonal antibody has been tested for use in western blotting, immunoprecipitation and immunofluorescence.  Specific conditions for reactivity should be optimized by the end user.  Expect bands approximately 115 and 105 in size corresponding to isoforms of HEF1 protein by western blotting in the appropriate cell lysate or extract.  This antibody does not recognize p130Cas.  Sin1 has not been tested.  IF was performed using 4% PFA fixed cells.  This monoclonal antibody mostly detects HEF1 localized at the focal adhesion sites.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
  • Pugacheva, Golemis: "The focal adhesion scaffolding protein HEF1 regulates activation of the Aurora-A and Nek2 kinases at the centrosome." dans: Nature cell biology, Vol. 7, Issue 10, pp. 937-46, (2005) (PubMed).

  • Antigène
    NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))
    Autre désignation
    HEF1 (NEDD9 Produits)
    Synonymes
    anticorps NEDD9, anticorps CAS-L, anticorps CAS2, anticorps CASL, anticorps CASS2, anticorps HEF1, anticorps Cas-L, anticorps CasL, anticorps E230025G09Rik, anticorps neural precursor cell expressed, developmentally down-regulated 9, anticorps neural precursor cell expressed, developmentally down-regulated gene 9, anticorps NEDD9, anticorps Nedd9
    Sujet
    HEF1, also known as Enhancer of filamentation 1, CRK-associated substrate-related protein, CAS-L, CasL, p105 and Neural precursor cell expressed developmentally down-regulated 9 is the product of the NEDD9 (CASGL) gene.  HEF1 functions as a docking protein that plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. HEF1 may also function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation.  HEF1 may also play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells.  Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.  HEF1 forms a homodimer and can heterodimerize with HLH proteins ID2, E12, E47 and also with p130cas.   HEF1 also forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK), adapter protein CRKL and LYN kinase and also interacts with MICAL and TXNL4/DIM1.  This protein localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts, it is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells, it is localized predominantly in the cell periphery with particular concentration in lamellipodia, but it is also found in the nucleus.  HEF1 is widely expressed although higher levels are detected in kidney, lung, and placental tissue.  HEF1 is also detected in T-cells, B-cells and diverse cell lines.  HEF1 is activated upon induction of cell growth.  Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55.  Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle.  Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. The p65 isoform is poorly detected.  Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with the mitotic spindle.
    Synonyms: Cas like docking antibody, CASL antibody, Crk associated substrate related protein antibody, dJ49G10.2 antibody, dJ761I2.1 antibody, Enhancer of filamentation 1 antibody
    ID gène
    4739, 5453680
    UniProt
    Q14511
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