VDAC1 anticorps (Internal Region)

N° du produit ABIN129654

Détail du produit anti-VDAC1 anticorps

Antigène: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Épitope: Internal Region

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VDAC1 est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Anti-VDAC/Porin Antibody is directed against human VDAC1/Porin1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).

Attributs du produit: VDAC/Porin Antibody recognizes VDAC (also known as Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL) which is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger - see below). The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mito-chondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest. This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations.

Stérilité: Sterile filtered

Immunogène: VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 185-197 of Human VDAC1/Porin1.
Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application: VDAC/Porin Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30-33 kDa in size corresponding to VDAC/Porin by western blotting in the appropriate cell lysate or extract.

Commentaires:

Gene Name: VDAC1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Références pour anticorps anti-VDAC1 (ABIN129654)

Thakurela, Garding, Jung, Müller, Goebbels, White, Werner, Tiwari: "The transcriptome of mouse central nervous system myelin." dans: Scientific reports, Vol. 6, pp. 25828, (2018) (PubMed).

Petermann, Haase, Knebel-Mörsdorf: "Impact of Rac1 and Cdc42 signaling during early herpes simplex virus type 1 infection of keratinocytes." dans: Journal of virology, Vol. 83, Issue 19, pp. 9759-72, (2009) (PubMed).

Bourekas, Slivka, Shah, Sunshine, Suarez: "Intraarterial thrombolytic therapy within 3 hours of the onset of stroke." dans: Neurosurgery, Vol. 54, Issue 1, pp. 39-44; discussion 44-6, (2003) (PubMed).

Détails sur VDAC1

Antigène: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Autre désignation: VDAC/Porin (VDAC1 Produits)

Synonymes: anticorps vdac1, anticorps VDAC1, anticorps 5076, anticorps ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 1, anticorps ATVDAC1, anticorps T22N4.9, anticorps T22N4_9, anticorps voltage dependent anion channel 1, anticorps PORIN, anticorps VDAC-1, anticorps AL033343, anticorps Vdac5, anticorps fa13f11, anticorps wu:fa13f11, anticorps zgc:85830, anticorps voltage-dependent anion channel 1 S homeolog, anticorps voltage-dependent anion channel 1, anticorps voltage dependent anion channel 1, anticorps vdac1.S, anticorps vdac1, anticorps VDAC1, anticorps Vdac1

Sujet: VDAC/Porin Antibody recognizes VDAC (also known as Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL) which is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger - see below). The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mito-chondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest. This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations.

ID gène: 130683

UniProt: Q9Y277