ECT2 anticorps (pThr790)

N° du produit ABIN129688

Détail du produit anti-ECT2 anticorps

Antigène: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Épitope: AA 785-795, pThr790

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ECT2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Réactivité croisée: Souris, Rat (Rattus), Poisson zebré (Brachydanio rerio), Chimpanzé, Poulet, Chien

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 785-795 of human ECT2 protein.

Isotype: IgG

Information d'application

Indications d'application: This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 101 kDa in size by western blotting in the appropriate cell lysate or extract.  Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is believed to be phospho specific for ECT2 phosphorylated at the pT790 residue.  Experiments to generate more conclusive evidence are currently under preparation.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur ECT2

Antigène: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Autre désignation: ECT2 (ECT2 Produits)

Synonymes: anticorps ECT2, anticorps arhgef31, anticorps xect2, anticorps AI528536, anticorps mKIAA4037, anticorps ARHGEF31, anticorps ect2, anticorps epithelial cell transforming 2, anticorps ect2 oncogene, anticorps epithelial cell transforming 2 S homeolog, anticorps ECT2, anticorps ect2, anticorps Ect2, anticorps ect2.S

Sujet: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. ECT2, also known as epithelial cell transforming sequence 2 oncogene, was originally isolated as a transforming gene from epithelial cells. ECT2 catalyzes guanine nucleotide exchange on the small GTPases, RhoA, Rac1, and Cdc42.  ECT2 may be phos-phorylated during G₂ and M phases, and phosphorylation may be required for its exchange activity. Unlike other known guanine nucleotide exchange factors for Rho GTPases, ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis. Expression of dominant-negative ECT2 or microinjection of affinity-purified anti-ECT2 antibody into interphase cells strongly inhibits cytokinesis. These results suggest that ECT2 is an important link between the cell cycle machinery and Rho signaling pathways involved in the regulation of cell division.  Phosphorylation at T790 or S375 significantly affects the catalytic activity of ECT2.
Synonyms: ECT 2 antibody, ECT2 protein antibody, Epithelial cell transforming sequence 2 antibody

ID gène: 1894, 21735572

UniProt: Q9H8V3

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization