VCP anticorps (FITC)
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- Antigène Voir toutes VCP Anticorps
- VCP (Valosin Containing Protein (VCP))
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Reactivité
- Humain, Souris
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp VCP est conjugé à/à la FITC
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Application
- Flow Cytometry (FACS)
- Specificité
- The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
- Réactivité croisée (Details)
- Mouse, Human
- Purification
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogène
- Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
- Clone
- Hs-14
- Isotype
- IgM
- Top Product
- Discover our top product VCP Anticorps primaire
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- Indications d'application
- Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 50 tests. Intraacrosomal staining.
- Commentaires
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The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
- Restrictions
- For Research Use only
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- Reconstitution
- No reconstitution is necessary.
- Buffer
- Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Do not freeze.
Avoid prolonged exposure to light. - Stock
- 4 °C
- Stockage commentaire
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis." dans: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 53, Issue 1, pp. 42-9, (2005) (PubMed).
: "[Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization]." dans: Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, Vol. 65, Issue 1, pp. 28-32, (2000) (PubMed).
: "
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Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis." dans: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 53, Issue 1, pp. 42-9, (2005) (PubMed).
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- Antigène
- VCP (Valosin Containing Protein (VCP))
- Autre désignation
- VCP (VCP Produits)
- Synonymes
- anticorps ALS14, anticorps IBMPFD, anticorps TERA, anticorps p97, anticorps CDC48, anticorps wu:fd16d05, anticorps wu:fj63d11, anticorps p93, anticorps 3110001E05, anticorps p97/VCP, anticorps 22.26, anticorps 7.12, anticorps Apple, anticorps BcDNA.GM02885, anticorps BcDNA:GM02885, anticorps CG2331, anticorps DmTER94, anticorps Dmel\CG2331, anticorps I, anticorps Ter94, anticorps Tera, anticorps VCP, anticorps VCP ATPase, anticorps VCP/p97, anticorps Vcp, anticorps anon-WO2004063362.65, anticorps anon-WO2004063362.67, anticorps cdc48, anticorps dTERA, anticorps dVCP, anticorps dmTERA, anticorps dvcp, anticorps l(2)03775, anticorps l(2)46CFf, anticorps l(2)46CFs, anticorps l(2)46Ch, anticorps pTER94, anticorps ter94, anticorps vcp, anticorps FANCG, anticorps valosin containing protein, anticorps valosin containing protein S homeolog, anticorps valosin-containing protein, anticorps CG2331 gene product from transcript CG2331-RA, anticorps zgc:136908, anticorps transitional endoplasmic reticulum ATPase, anticorps VCP, anticorps vcp.S, anticorps vcp, anticorps Vcp, anticorps Bm1_56585, anticorps LOC100136246, anticorps TER94, anticorps zgc:136908, anticorps LOC101109680
- Sujet
- Valosin containing protein,VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,TERA, CDC48
- ID gène
- 7415
- UniProt
- P55072
- Pathways
- ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway
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