CD4 anticorps

N° du produit ABIN133174

Détail du produit anti-CD4 anticorps

Antigène: CD4

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD4 est non-conjugé

Application: Flow Cytometry (FACS)

Specificité: Rat CD4

Purification: Purified from ascitic fluid via Protein G Chromatography

Immunogène: Rat thymocyte membrane . Fusion Partner: P3-NSI/1-Ag 4 . Donor: BALB/c spleen

Clone: W3-25

Isotype: IgG1

Information d'application

Indications d'application: This clone is reported to work with frozen and paraffin sections (11) and functional testing (12).

Restrictions: For Research Use only

Stockage

Concentration: 0.5 µg/10 cells

Buffer: Purified Ig buffered in PBS and 0.02% NaN (Purified from ascitic fluid via Protein G Chromatography).

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-CD4 (ABIN133174)

Saravanan, Sareen, Abu-El-Rub, Ashour, Sequiera, Ammar, Gopinath, Shamaa, Sayed, Moudgil, Vadivelu, Dhingra: "Graphene Oxide-Gold Nanosheets Containing Chitosan Scaffold Improves Ventricular Contractility and Function After Implantation into Infarcted Heart." dans: Scientific reports, Vol. 8, Issue 1, pp. 15069, (2019) (PubMed).

Détails sur CD4

Antigène: CD4

Autre désignation: CD4 (CD4 Produits)

Synonymes: anticorps cd4, anticorps L3T4, anticorps Ly-4, anticorps CD4mut, anticorps W3/25, anticorps p55, anticorps CD4 molecule, anticorps carcinoembryonic antigen-related cell adhesion molecule 5, anticorps T-cell surface glycoprotein CD4, anticorps CD4 antigen, anticorps Cd4 molecule, anticorps CD4, anticorps LOC100125525, anticorps cd4, anticorps Cd4

Sujet: Anti-rat T helper cell monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells and peritoneal macrophages. (1,2,3,10.) The antigen recognized by this antibody is a surface glycoprotein of Mr 48 000 - 52 000 and is the homologue of the human CD4 and the mouse L3/T4 antigen. ABIN133174labels the rat T helper subset, which mediates the helper activity for B and T cells, graft vs. host (GVH) reactivity and produces IL-2 in the mixed lymphocyte reaction (MLR). (2,4,6) Addition of ABIN133174to the MLR, inhibited proliferation and blocks the production of IL- 2. (4,5,6,9) T cells which mediate cytotoxicity and suppressor functions are not labelled. (Thus, cells labelled by this antibody are not labelled by MRC OX-8.) ABIN133174is invaluable for separating T cell subsets for functional studies and for labelling cells in tissue sections. It has been used in studying the role of T lymphocytes in graft rejections (7) and in studying the subsets of T cells in the rat which mediate graft vs. host disease. (8) This particular antibody is also one of three antibodies which labels T lymphocyte populations in the rat. These clones include W3/13 ABIN133174, which labels all T cells, as well as MRC OX-8 ABIN133174 and W3/25 ABIN133174 which label non-overlapping T cell subpopulations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (11) and functional testing (12).

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events