CLEC14A anticorps (AA 260-310)

N° du produit ABIN1385496

Détail du produit anti-CLEC14A anticorps

Antigène: CLEC14A (C-Type Lectin Domain Family 14, Member A (CLEC14A))

Épitope: AA 260-310

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CLEC14A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human EGFR5/CLEC14A

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Independent Validation (IHC (fp))

Validation #100004 (Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections))

by: Memorial Sloan-Kettering Cancer Center, Parada lab

No.: #100004

Date: 23.11.2015

Antigène: CLEC14A

Application validée: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Contrôle positif: Tumor cell transplanted mouse brain was dissected

Validation Images

IF validation of CLEC14A antibody ABIN1385496 on mouse brain tumor sections. A. ABIN1385496 diluted 1:100 (blue), DAPI counterstaining (red). B. Dilution factor 1:100. C. Dilution factor 1:200. D. Dilution factor 1:400.

Protocole

Anticorps primaire: ABIN1385496

Anticorps secondaire: Donkey-anti-Rabbit-Cy3

Full Protocol:

• Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
• Immunostaining for Cryo Sections:
• Dry slides at 50-55°C for 15-30 min (or RT for 30min)
• Wash three times with PBS 5min (shake gently)
• Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
• Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
• Rabbit-anti-CLEC14A antibody (ABIN1385496) diluted 1:50-1:400
• Wash three times with PBS 5min (shake gently)
• Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
• Donkey-anti-Rabbit-Cy3
• DAPI 1:100
• Wash three times with PBS 5min (shake gently)
• Add three drops of water-based Immu-mount
• Cover with 24x60 cover glass
• Imaging
• Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Notes: ABIN1385496 worked within the diution range 1:50 - 1:200; the antibody did not work well at a 1:400 dilution.

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur CLEC14A

Antigène: CLEC14A (C-Type Lectin Domain Family 14, Member A (CLEC14A))

Autre désignation: EGFR5 (CLEC14A Produits)

Synonymes: anticorps C14orf27, anticorps CEG1, anticorps EGFR-5, anticorps 1200003C23Rik, anticorps AI642649, anticorps C-type lectin domain containing 14A, anticorps C-type lectin domain family 14, member a, anticorps CLEC14A, anticorps Clec14a

Sujet:

Synonyms: CEG1, EGFR-5, C14orf27, C-type lectin domain family 14 member A, Epidermal growth factor receptor 5, CLEC14A, EGFR5, UNQ236/PRO269

Background: This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This family member plays a role in cell-cell adhesion and angiogenesis. It functions in filopodia formation, cell migration and tube formation. Due to its presence at higher levels in tumor endothelium than in normal tissue endothelium, it is considered to be a candidate for tumor vascular targeting.

ID gène: 161198

UniProt: Q86T13