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E-cadherin anticorps (AA 401-500)

CDH1 Reactivité: Humain, Souris, Rat WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1387847
  • Antigène Voir toutes E-cadherin (CDH1) Anticorps
    E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
    Épitope
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 8
    • 8
    • 7
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 401-500
    Reactivité
    • 250
    • 102
    • 86
    • 18
    • 17
    • 16
    • 9
    • 9
    • 9
    • 6
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 201
    • 83
    • 8
    • 7
    Lapin
    Clonalité
    • 187
    • 111
    • 1
    Polyclonal
    Conjugué
    • 140
    • 36
    • 29
    • 10
    • 10
    • 9
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp E-cadherin est non-conjugé
    Application
    • 206
    • 93
    • 92
    • 67
    • 57
    • 56
    • 38
    • 34
    • 28
    • 26
    • 17
    • 15
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))
     Réactivité croisée
    Humain, Souris, Rat
    Homologie
    Cow,Pig,Horse,Rabbit
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human E-cadherin
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Restrictions
    For Research Use only
  • Validation #029768 (Flow Cytometry)
    'Independent Validation' signe
    by
    Flow Cytometry & Cell Separation Facility, Purdue University
    No.
    #029768
    Date
    22.07.2014
    Antigène
    Numéro du lot
    130902
    Application validée
    Flow Cytometry
    Contrôle positif
    MCF-7 cells
    Contrôle négative
    SH-SY5Y cells
    Conclusion
    A weak but specific signal is observed in the positive control MCF7 cells stained with anti-E-Cadherin plus secondary antibody compared with isotype, secondary only and unstained cells. No staining was observed in the negative control SH-SY5Y cells as expected.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antigen: Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1)
    • Catalog number: ABIN1387847
    • Lot number: 130902
    • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
    Anticorps secondaire
    • Antibody: Goat anti-rabbit IgG-Alexa 647
    • Dilution: 1:500 in 1X PBS containing 0.5% BSA
    Full Protocol
    • Positive and negative control cells were cultured in DMEM + 10% FBS. - Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
    • Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
    • Cells were incubated for 30 min on ice.
    • Labeled cells were washed twice in PBS containing 0.5% BSA.
    • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
    • Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
    • Labeled cells were washed twice in PBS containing 0.5% BSA.
    • Propidium Iodide (PI) was added to discern live cells from dead cells.
    • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
    Notes
    - The data displayed is gated on PI negative cells.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Guo, Gao, Sui, Jiao, Sun, Fu, Jin: "miR-375-3p/YWHAZ/β-catenin axis regulates migration, invasion, EMT in gastric cancer cells." dans: Clinical and experimental pharmacology & physiology, Vol. 46, Issue 2, pp. 144-152, (2019) (PubMed).

    Yun, Gao, Yue, Guo, Li, Sang: "Sulfate Aerosols Promote Lung Cancer Metastasis by Epigenetically Regulating the Epithelial-to-Mesenchymal Transition (EMT)." dans: Environmental science & technology, Vol. 51, Issue 19, pp. 11401-11411, (2018) (PubMed).

    Fang, Liu, Wu, Liu, Pan, Li: "Upregulation of long noncoding RNA CCAT1-L promotes epithelial-mesenchymal transition in gastric adenocarcinoma." dans: OncoTargets and therapy, Vol. 11, pp. 5647-5655, (2018) (PubMed).

    Lin, Zhang, Dai, Zhang, Zhang, Xue, Wu: "TFF3 Contributes to Epithelial-Mesenchymal Transition (EMT) in Papillary Thyroid Carcinoma Cells via the MAPK/ERK Signaling Pathway." dans: Journal of Cancer, Vol. 9, Issue 23, pp. 4430-4439, (2018) (PubMed).

    Wang, Nikhil, Viccaro, Chang, White, Shah: "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." dans: BMC biology, Vol. 15, Issue 1, pp. 10, (2017) (PubMed).

    Borin, Shankar, Angara, Rashid, Jain, Iskander, Ara, Lebedyeva, Korkaya, Achyut, Arbab: "HET0016 decreases lung metastasis from breast cancer in immune-competent mouse model." dans: PLoS ONE, Vol. 12, Issue 6, pp. e0178830, (2017) (PubMed).

    Liu, Xiao: "Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia." dans: BMC ophthalmology, Vol. 17, Issue 1, pp. 135, (2017) (PubMed).

    Wang, Nikhil, Viccaro, Chang, Jacobsen, Sandusky, Shah: "The Aurora-A-Twist1 axis promotes highly aggressive phenotypes in pancreatic carcinoma." dans: Journal of cell science, Vol. 130, Issue 6, pp. 1078-1093, (2017) (PubMed).

    Short, Kasper, van der Aa, Andeweg, Zaaraoui-Boutahar, Goeijenbier, Richard, Herold, Becker, Scott, Limpens, Koster, Bárcena, Fouchier, Kirkpatrick, Kuiken: "Influenza virus damages the alveolar barrier by disrupting epithelial cell tight junctions." dans: The European respiratory journal, Vol. 47, Issue 3, pp. 954-66, (2016) (PubMed).

    Hu, Duan, Li, Wang, Wang, Chu, Sun, Liu, Wang, Lu, Wen: "Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-β1-Induced Epithelial-to-Mesenchymal Transition." dans: PLoS ONE, Vol. 11, Issue 4, pp. e0153409, (2016) (PubMed).

    Neelam, Brooks, Cammarata: "Lenticular cytoprotection, part 2: link between glycogen synthase kinase-3?, epithelial to mesenchymal transition, and mitochondrial depolarization." dans: Molecular vision, Vol. 20, pp. 1758-75, (2015) (PubMed).

    Jayachandran, Lugo, Heiling, Miller, Rule, Lieske: "Extracellular vesicles in urine of women with but not without kidney stones manifest patterns similar to men: a case control study." dans: Biology of sex differences, Vol. 6, pp. 2, (2015) (PubMed).

    Chen, Cheng, Chen, Sue, Liu, Cheng, Hsu, Chen: "MicroRNA-328 inhibits renal tubular cell epithelial-to-mesenchymal transition by targeting the CD44 in pressure-induced renal fibrosis." dans: PLoS ONE, Vol. 9, Issue 6, pp. e99802, (2014) (PubMed).

    Cai: "Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma." dans: Experimental and therapeutic medicine, Vol. 6, Issue 6, pp. 1489-1493, (2013) (PubMed).

  • Antigène
    E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
    Autre désignation
    E cadherin (CDH1 Produits)
    Synonymes
    anticorps Arc-1, anticorps CD324, anticorps CDHE, anticorps ECAD, anticorps LCAM, anticorps UVO, anticorps E-cadherin, anticorps cdh1, anticorps si:dz180o5.2, anticorps uvo, anticorps lcam, anticorps E-Cad, anticorps XBcad, anticorps l-cam, anticorps xcdh1, anticorps cdhc-A, anticorps xb-cad, anticorps XTCAD-1, anticorps c-cadherin, anticorps cadherin-1, anticorps uvomorulin, anticorps XB-cadherin, anticorps arc-1, anticorps cd324, anticorps cdhe, anticorps ecad, anticorps AA960649, anticorps E-cad, anticorps Ecad, anticorps L-CAM, anticorps Um, anticorps cadherin 1, anticorps si:busm1-180o5.2, anticorps cadherin 1, type 1, E-cadherin (epithelial), anticorps cadherin 1, type 1 S homeolog, anticorps CDH1, anticorps si:busm1-180o5.2, anticorps cdh1, anticorps Cdh1, anticorps cdh1.S
    Sujet

    Synonyms: UVO, CDHE, ECAD, LCAM, Arc-1, CD324, Cadherin-1, CAM 12/8, Epithelial cadherin, E-cadherin, Uvomorulin, CDH1

    Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

    ID gène
    999
    UniProt
    P12830
    Pathways
    Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
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