Influenza Nucleoprotein anticorps (Influenza A Virus (A/Russia:St.Petersburg/8/2006)) (AA 71-170) (HRP)

N° du produit ABIN1415073

Détail du produit

Antigène: Influenza Nucleoprotein (NP)

Épitope: AA 71-170

Reactivité: Influenza A Virus, Virus

Virus Strain: A/Russia:St.Petersburg/8/2006

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: HRP

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Réactivité croisée: Virus

Réactivité croisée (Details): Influenza A virus

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from Influenza A virus (strain A/Russia:St.Petersburg/8/2006 H1N1) Nucleoprotein

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Référence

Chen, Angel, Li, Finch, Gonzalez, Sutton, Santos, Perez: "All-in-one bacmids: an efficient reverse genetics strategy for influenza A virus vaccines." dans: Journal of virology, Vol. 88, Issue 17, pp. 10013-25, (2014) (PubMed).

Détail du antigène

Antigène: Influenza Nucleoprotein (NP)

Autre désignation: Influenza A virus Nucleoprotein (NP Produits)

Synonymes: anticorps nucleoprotein, anticorps NP

Classe de substances: Influenza Protein

Sujet:

Synonyms: Nucleoprotein, NP, Nucleocapsid protein, Protein N, Influenza A virus H1N1, H3N2 Nucleoprotein, H9N2 Nucleoprotein, H2N2 Nucleoprotein, H3N8 Nucleoprotein, H7N7 Nucleoprotein, H5N1 Nucleoprotein.

Background: Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.