CNTF anticorps

N° du produit ABIN1497554

Détail du produit anti-CNTF anticorps

Antigène: CNTF (Ciliary Neurotrophic Factor (CNTF))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CNTF est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human CNTF

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human CNTF.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B8208 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human CNTF (1 μg/mL) a concentration of 7-11 μg/mL of this antibody is required. ELISA: To detect hCNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CNTF (LS-C104338) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCNTF. Western Blot: To detect hCNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCNTF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur CNTF

Antigène: CNTF (Ciliary Neurotrophic Factor (CNTF))

Autre désignation: CNTF (CNTF Produits)

Synonymes: anticorps GPA, anticorps CNTF, anticorps HCNTF, anticorps AI429687, anticorps ciliary neurotrophic factor, anticorps CNTF, anticorps Cntf

Sujet: Name/Gene ID: CNTF

Synonyms: CNTF, Ciliary neurotrophic factor, HCNTF

ID gène: 1270

UniProt: P26441

Pathways: Signalistation JAK/STAT