p53 anticorps (Tumor Protein P53) (AA 14-389)

Details for Product anti-TP53 Antibody No. ABIN152249
Antigène
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
Épitope
AA 14-389
134
80
79
72
66
66
66
59
58
54
51
45
41
41
37
30
28
24
22
21
21
18
17
16
15
15
15
15
15
15
14
14
13
13
12
11
11
10
10
9
9
9
8
8
8
8
8
7
7
7
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus), Yeast (Saccharomyces cerevisiae)
2345
501
466
249
100
64
63
32
26
22
22
17
16
7
6
6
5
4
4
4
3
3
3
2
2
1
1
1
Hôte
Souris
1648
770
7
5
1
1
1
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp p53 est non-conjugé
89
62
48
41
40
40
40
39
39
38
33
32
14
12
12
12
12
11
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
5
2
2
2
Application
Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1836
766
747
574
436
413
370
155
109
103
99
89
75
41
32
16
15
15
13
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
Immunogène Gel-purified p53-beta-galactosidase fusion protein containing murine p53 from aa 14-389 (derived from pSV53C cDNA clone).
Clone PAb 240
Isotype IgG1 kappa
Specificité This monoclonal recognizes both mutant forms and wild-type human p53 under denaturing conditions.
Aucune reactivité croisée Xenopus laevis
Purification Protein G purified
Plasmids, Primers & others Plasmids, Primers & others p53 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation p53 (TP53 Antibody Extrait)
Sujet Gene Symbol: TP53
ID gène 7157
Pathways Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy
Indications d'application Western Blot 1:1000-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunohistochemistry 1:250-1:500, Immunocytochemistry/Immunofluorescence 5 μg/mL, Immunoprecipitation 10 μg/mg, Immunohistochemistry-Paraffin 1:250-1:500, Immunohistochemistry-Frozen, CyTOF-readyAntigen retrieval with IHC-P is not essential but may optimise staining. In IP this antibody reacts with only mutant p53 protein under non-denaturing conditions. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 1394225) Use in Immunohistochemistry-Frozen reported in various pieces of scientific literature.
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock 4 °C,-20 °C
Stockage commentaire Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Images (Fournisseur)
 image for anti-Tumor Protein P53 (TP53) (AA 14-389) antibody (ABIN152249) Flow (Intracellular): p53 Antibody (PAb 240) [ABIN152249] - An intracellular stain wa...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Protein P53 (TP53) (AA 14-389) antibody (ABIN152249) Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [ABIN152249] - p53 was detected...
Immunofluorescence (IF) image for anti-Tumor Protein P53 (TP53) (AA 14-389) antibody (ABIN152249) Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [ABIN152249] - HeLa ce...
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 14-389) antibody (ABIN152249) Western Blot: p53 Antibody (PAb 240) [ABIN152249] - WB analysis of p53 in MCF7 and He...
Flow Cytometry (FACS) image for anti-Tumor Protein P53 (TP53) (AA 14-389) antibody (ABIN152249) Flow Cytometry: p53 Antibody (PAb 240) - An intracellular stain was performed on A54...
Produit citée dans: Yang, Zhao, Feng, Shi, Jiang, Liang, Sun, Xu, Duan, Sun: "PM2.5-induced ADRB2 hypermethylation contributed to cardiac dysfunction through cardiomyocytes apoptosis via PI3K/Akt pathway." dans: Environment international, Vol. 127, pp. 601-614, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Graham, Minasyan, Lomova, Cass, Balanis, Friedman, Chan, Zhao, Delgado, Go, Beck, Hurtz, Ng, Qiao, Ten Hoeve, Palaskas, Wu, Müschen, Multani, Port, Larson, Schultz, Braas, Christofk, Mellinghoff et al.: "Recurrent patterns of DNA copy number alterations in tumors reflect metabolic selection pressures. ..." dans: Molecular systems biology, Vol. 13, Issue 2, pp. 914, 2017 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Mouse (Murine)).

Shi, Ma, Zhou, Fu: "Renal Protective Effects of 17β-Estradiol on Mice with Acute Aristolochic Acid Nephropathy." dans: Molecules (Basel, Switzerland), Vol. 21, Issue 10, 2016 (PubMed).

Ge, Pettan-Brewer, Morton, Carter, Fatemi, Rabinovitch, Ladiges: "Mitochondrial catalase suppresses naturally occurring lung cancer in old mice." dans: Pathobiology of aging & age related diseases, Vol. 5, pp. 28776, 2015 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Immunohistochemistry

Biswas, Mitchell, Johnson: "E2F1 responds to ultraviolet radiation by directly stimulating DNA repair and suppressing carcinogenesis." dans: Cancer research, Vol. 74, Issue 12, pp. 3369-77, 2014 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Immunohistochemistry (Paraffin-embedded Sections)

Lange, Bedford, Reh, Wittschieben, Carbajal, Kusewitt, DiGiovanni, Wood: "Dual role for mammalian DNA polymerase ζ in maintaining genome stability and proliferative responses." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 8, pp. E687-96, 2013 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Immunohistochemistry (Paraffin-embedded Sections)

Jensen, Factor, Fantozzi, Helin, Huh, Thorgeirsson: "Reduced hepatic tumor incidence in cyclin G1-deficient mice." dans: Hepatology (Baltimore, Md.), Vol. 37, Issue 4, pp. 862-70, 2003 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Western Blotting

Asher, Lotem, Cohen, Sachs, Shaul: "Regulation of p53 stability and p53-dependent apoptosis by NADH quinone oxidoreductase 1." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 3, pp. 1188-93, 2001 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Western Blotting

Jones, Turner, McIlwrath, Brown, Dive: "Cisplatin- and paclitaxel-induced apoptosis of ovarian carcinoma cells and the relationship between bax and bak up-regulation and the functional status of p53." dans: Molecular pharmacology, Vol. 53, Issue 5, pp. 819-26, 1998 (PubMed). (Échantillon (espèces): Human). Détails: Immunoprecipitation

Blagosklonny, Toretsky, Bohen, Neckers: "Mutant conformation of p53 translated in vitro or in vivo requires functional HSP90." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 16, pp. 8379-83, 1996 (PubMed). (Échantillon (espèces): Yeast). Détails: Immunoprecipitation

Bargonetti, Manfredi, Chen, Marshak, Prives: "A proteolytic fragment from the central region of p53 has marked sequence-specific DNA-binding activity when generated from wild-type but not from oncogenic mutant p53 protein." dans: Genes & development, Vol. 7, Issue 12B, pp. 2565-74, 1994 (PubMed). (Échantillon (espèces): Human). Détails: Western Blotting

Rubio, von Deimling, Yandell, Wiestler, Gusella, Louis: "Accumulation of wild type p53 protein in human astrocytomas." dans: Cancer research, Vol. 53, Issue 15, pp. 3465-7, 1993 (PubMed). (Échantillon (espèces): Human). Détails: Immunohistochemistry (Frozen Sections)

Halazonetis, Davis, Kandil: "Wild-type p53 adopts a 'mutant'-like conformation when bound to DNA." dans: The EMBO journal, Vol. 12, Issue 3, pp. 1021-8, 1993 (PubMed). (Échantillon (espèces): Human). Détails: Western Blotting,Immunoprecipitation

Eccles, Brett, Lessells, Gruber, Lane, Steel, Leonard: "Overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma." dans: British journal of cancer, Vol. 65, Issue 1, pp. 40-4, 1992 (PubMed). (Échantillon (espèces): Human). Détails: Immunohistochemistry (Frozen Sections)

Somers, Merrick, Lopez, Incognito, Schechter, Casey: "Frequent p53 mutations in head and neck cancer." dans: Cancer research, Vol. 52, Issue 21, pp. 5997-6000, 1992 (PubMed). (Échantillon (espèces): Human). Détails: Immunocytochemistry,Immunofluorescence

Remvikos, Tominaga, Hammel, Laurent-Puig, Salmon, Dutrillaux, Thomas: "Increased p53 protein content of colorectal tumours correlates with poor survival." dans: British journal of cancer, Vol. 66, Issue 4, pp. 758-64, 1992 (PubMed). (Échantillon (espèces): Human). Détails: ELISA,Flow Cytometry

Cunningham, Lust, Schaid, Bren, Carpenter, Rizza, Kovach, Thibodeau: "Expression of p53 and 17p allelic loss in colorectal carcinoma." dans: Cancer research, Vol. 52, Issue 7, pp. 1974-80, 1992 (PubMed). (Échantillon (espèces): Human). Détails: Immunohistochemistry

Farrell, Allan, Shanahan, Vousden, Crook: "p53 is frequently mutated in Burkitt's lymphoma cell lines." dans: The EMBO journal, Vol. 10, Issue 10, pp. 2879-87, 1991 (PubMed). Détails: Immunoprecipitation

Martinez, Georgoff, Martinez, Levine: "Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein." dans: Genes & development, Vol. 5, Issue 2, pp. 151-9, 1991 (PubMed). (Échantillon (espèces): Rat (Rattus)). Détails: Immunocytochemistry,Immunofluorescence

Davidoff, Humphrey, Iglehart, Marks: "Genetic basis for p53 overexpression in human breast cancer." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 11, pp. 5006-10, 1991 (PubMed). (Échantillon (espèces): Human). Détails: Immunohistochemistry

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