p53 anticorps (Tumor Protein P53) (AA 50-100)

Details for Product anti-TP53 Antibody No. ABIN152309
Antigène
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
Épitope
AA 50-100
134
80
79
72
66
66
66
59
58
54
51
45
41
41
37
30
28
24
22
21
21
18
17
16
15
15
15
15
15
15
14
14
13
13
12
11
11
10
10
9
9
9
8
8
8
8
8
7
7
7
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain
2345
501
466
249
100
64
63
32
26
22
22
17
16
7
6
6
5
4
4
4
3
3
3
2
2
1
1
1
Hôte
Lapin
1648
770
7
5
1
1
1
1
Clonalité
Polyclonal
Conjugué
Cet anticorp p53 est non-conjugé
89
62
48
41
40
40
40
39
39
38
33
32
14
12
12
12
12
11
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
5
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
1836
766
747
574
436
413
370
155
109
103
99
89
75
41
32
16
15
15
13
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
Immunogène The immunogen this antibody was made to, maps to a region between residues 50-100 of human tumor protein p53 using the SwissProt entry P04637 (GeneID 7157).
Isotype IgG
Specificité This is specific for human p53.
Purification Immunogen affinity purified
Plasmids, Primers & others Plasmids, Primers & others p53 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation p53 (TP53 Antibody Extrait)
Sujet Gene Symbol: TP53
ID gène 7157
UniProt P04637
Pathways Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy
Indications d'application Western Blot 1:5000-1:25000, Flow Cytometry 1.5 μg/10^6 cells in 150 μL, Immunohistochemistry 1:1000 - 1:5000, Immunocytochemistry/Immunofluorescence 1:500 - 1:2500, Immunoprecipitation 2 - 10 μg/mg lysate, Immunohistochemistry-Paraffin 1:1000 - 1:5000, Proximity Ligation Assay 1:1000-1:20000, Chromatin Immunoprecipitation (ChIP) 4 μg/30 μgImmunohistochemistry 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ChIP-Seq 4 μg/30 μg chromatin
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Do not freeze.
Stock 4 °C
Stockage commentaire Store at 4°C. Do not freeze.
Images (Fournisseur)
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) anti-Tumor Protein P53 (TP53) (AA 50-100) antibody
Flow Cytometry (FACS) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (Image 2)
Immunoprecipitation (IP) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Chromatin Immunoprecipitation (ChIP): p53 Antibody [ABIN152309] - Chromatin from MCF7...
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Western Blot: p53 Antibody [ABIN152309] - Whole cell lysate (50 ug) from HeLa and 293...
Flow Cytometry (FACS) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Flow Cytometry: p53 Antibody [ABIN152309] - Baseline p53 expression in human lymphocy...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Immunohistochemistry-Paraffin: p53 Antibody [ABIN152309] - Human skin carcinoma. Anti...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate In Situ PLA...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Western Blot: p53 Antibody - Detection of Human p53 by Western Blot. Samples: Whole ...
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Western Blot: p53 Antibody - Whole cell lysate (5, 15 and 50 ug) from 293T cells pre...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Immunohistochemistry-Paraffin: p53 Antibody - FFPE section of human lung cancer Anti...
Produit citée dans: Wan, Block, Scribano, Thiry, Esbona, Audhya, Weaver: "Mad1 destabilizes p53 by preventing PML from sequestering MDM2." dans: Nature communications, Vol. 10, Issue 1, pp. 1540, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Spurlock, Tossberg, Matlock, Olsen, Aune: "Methotrexate inhibits NF-κB activity via long intergenic (noncoding) RNA-p21 induction." dans: Arthritis & rheumatology (Hoboken, N.J.), Vol. 66, Issue 11, pp. 2947-57, 2014 (PubMed).

Spurlock, Gass, Bryant, Wells, Olsen, Aune: "Methotrexate-mediated inhibition of nuclear factor κB activation by distinct pathways in T cells and fibroblast-like synoviocytes." dans: Rheumatology (Oxford, England), Vol. 54, Issue 1, pp. 178-87, 2014 (PubMed).

Spurlock, Tossberg, Fuchs, Olsen, Aune: "Methotrexate increases expression of cell cycle checkpoint genes via JNK activation." dans: Arthritis and rheumatism, Vol. 64, Issue 6, pp. 1780-9, 2012 (PubMed). (Échantillon (espèces): Human). Détails: Flow Cytometry

Shultz, Saito, Najima, Tanaka, Ochi, Tomizawa, Doi, Sone, Suzuki, Fujiwara, Yasukawa, Ishikawa: "Generation of functional human T-cell subsets with HLA-restricted immune responses in HLA class I expressing NOD/SCID/IL2r gamma(null) humanized mice." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 29, pp. 13022-7, 2010 (PubMed).

Wang, Wang, Chang, Wu, Chao, Kao, Yuan, Lin, Yang, Chan, Li, Hong, Yang: "p53 controls cancer cell invasion by inducing the MDM2-mediated degradation of Slug." dans: Nature cell biology, Vol. 11, Issue 6, pp. 694-704, 2009 (PubMed).

Shao, Fujii, Colmegna, Oishi, Goronzy, Weyand: "Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis." dans: The Journal of experimental medicine, Vol. 206, Issue 6, pp. 1435-49, 2009 (PubMed).

Tsai, Tseng, Lin: "Two wobble-splicing events affect ING4 protein subnuclear localization and degradation." dans: Experimental cell research, Vol. 314, Issue 17, pp. 3130-41, 2008 (PubMed).

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