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c-MYC anticorps (AA 31-80)

MYC Reactivité: Humain, Souris, Rat WB, IHC, ELISA, IF Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1532205
  • Antigène Voir toutes c-MYC (MYC) Anticorps
    c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
    Épitope
    • 55
    • 45
    • 44
    • 40
    • 24
    • 21
    • 19
    • 16
    • 15
    • 11
    • 11
    • 11
    • 10
    • 7
    • 7
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 31-80
    Reactivité
    • 376
    • 170
    • 123
    • 15
    • 14
    • 13
    • 13
    • 13
    • 9
    • 9
    • 8
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 297
    • 99
    • 7
    • 3
    • 2
    • 1
    Lapin
    Clonalité
    • 288
    • 120
    Polyclonal
    Conjugué
    • 198
    • 32
    • 27
    • 26
    • 10
    • 9
    • 9
    • 9
    • 9
    • 9
    • 9
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp c-MYC est non-conjugé
    Application
    • 308
    • 142
    • 96
    • 83
    • 80
    • 66
    • 58
    • 28
    • 26
    • 17
    • 15
    • 14
    • 12
    • 11
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
    Specificité

    MYC Antibody detects endogenous levels of total MYC protein.

    Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
    Pureté
    > 95 %
    Immunogène
    The antiserum was produced against synthesized peptide derived from human MYC.
    Isotype
    IgG
    Top Product
    Discover our top product MYC Anticorps primaire
  • Indications d'application
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
    Commentaires

    Unigene-Number: Hs.202453 (NCBI Gene Symbol: MYC)

    Restrictions
    For Research Use only
  • Validation #102753 (Western Blotting)
    'Independent Validation' signe
    by
    Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University of Göttingen
    No.
    #102753
    Date
    03.07.2018
    Antigène
    MYC
    Numéro du lot
    210020 and 424170020
    Application validée
    Western Blotting
    Contrôle positif

    HEK-293 cells transfected with Myc expression plasmid

    Contrôle négative

    HEK-293 cells transfected with HA-Spag4-myc expression plasmid

    Conclusion

    Passed. ABIN1532205 detects Myc protein in a western blot. No cross-reaction was observed with the myc-tag sequence positioned at the C-terminus of a recombinant protein.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN1532205
    Anticorps secondaire
    rabbit anti-mouse IgG, HRP-linked (Sigma A9044, Lot 034M4761)
    Full Protocol
    • Grow HEK-293 cells (ATCC, CRL-1658) in DMEM+GlutaMAXDMEM+GlutaMAX (Gibco, 10567-014, lot 1922818) supplemented with fetal bovine serum (Gibco, 10500-064, lot 08FO477K) and Pen/Strep (Gibco, 15140-122, lot 1924797) at 37°C and 5% CO2 to 70% confluency.
    • Transfect cells either with a Myc expression plasmid (pcDNA3.1-Myc) or HA- and myc-tagged SPAG4 expression plasmids using EndofectinMax (GeneCopoeia) following the manufacturer´s instructions.
    • Grow cells for 24h.
    • Lyse cells in SDS-sample buffer and denature total cellular lysates for 5min at 95°C. Subsequently separate them on a denaturing 10% SDS-PAGE (Laemmli 1970).
    • Transfer proteins onto 0.2µm Protran membrane (GE Healthcare, 10600004, A10043108) by wet blotting for 1h at 400mA (Towbin et al., 1979).
    • Block the membrane in TBST (50mM Tris-HCl, pH7.4, 150mM NaCl, 0.2% Tween 20) containing 5% milk (blocking solution) for 60min at RT.
    • Incubate membrane with primary rabbit anti-myc antibody (antibodies-online, ABIN1532205 Lot#424170020 and Lot#210020) diluted 1:500 in blocking solution overnight at 4°C.
    • Wash membrane with TBST for 45min at RT.
    • Incubate membrane with secondary goat anti-rabbit IgG (H+L), HRP-linked (Jackson Immuno Research, 111-035-003, lot 123450) diluted 1:1000 in blocking solution for 45min at RT.
    • Wash membrane 6x for 5min with TBST.
    • Reveal protein bands using Clarity Max Western ECL substrate (Bio-Rad, 1705062) and capture images via Chemidoc Imaging System (BioRad).
    • Incubate membrane with primary mouse anti-HA-tag antibody (clone 12CA5) diluted 1:500 in blocking solution overnight at 4°C.
    • Wash membrane with TBST for 45min at RT.
    • Incubate membrane with secondary rabbit anti-mouse IgG, HRP-linked (Sigma A9044, Lot 034M4761) diluted 1:1000 in blocking solution for 45min at RT.
    • Reveal protein bands using Clarity Max Western ECL substrate (Bio-Rad, 1705062).
    Notes

    In lysates of HEK-293 cells ectopically expressing recombinant protein with an N-terminal HA- and a C-terminal myc-tag ABIN1532205 did not detect the labeled protein at the expected molecular weight. In contrast, an anti-HA-tag antibody (clone 12CA5) revealed a protein at the expected MW.

  • Validation #103023 (Immunocytochemistry)
    'Independent Validation' signe
    by
    Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University of Göttingen
    No.
    #103023
    Date
    03.07.2018
    Antigène
    MYC
    Numéro du lot
    210020 and 424170020
    Application validée
    Immunocytochemistry
    Contrôle positif

    HEK-293 cells transfected with Myc expression plasmid

    Contrôle négative

    HEK-293 cells transfected with HA-Spag4-myc expression plasmid

    Conclusion

    Passed. ABIN1532205 (lot 424170020 and lot 210020) detects Myc in immunocytochemistry. No cross-reaction was observed with the myc-tag sequence positioned at the C-terminus of a recombinant protein.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN1532205
    Anticorps secondaire
    goat anti-rabbit IgG MFP590 (Mobitec MFP-A1037, lot 3002088), F(ab')2-Goat anti-Rabbit IgG (H+L) Alexa Fluor 555 (Life Technologies, A21430, lot 1637266)
    Full Protocol
    • Grow HEK-293 cells (ATCC, CRL-1658) in DMEM+GlutaMAXDMEM+GlutaMAX (Gibco, 10567-014, lot 1922818) supplemented with fetal bovine serum (Gibco, 10500-064, lot 08FO477K) and Pen/Strep (Gibco, 15140-122, lot 1924797) at 37°C and 5% CO2 to 70% confluency.
    • Transfect cells either with a Myc expression plasmid (pcDNA3.1-Myc) or HA- and myc-tagged SPAG4 expression plasmids using EndofectinMax (GeneCopoeia) following the manufacturer´s instructions.
    • Grow cells for 24h.
    • Fix cells in 3.7% paraformaldehyde (in PBS) for 20min at 4°C followed by incubation in 0.3% Triton X-100 for 10min at 4°C.
    • Block cells in PBS containing 1% BSA and 0.5% Tween-20 (PBT) for 1h at RT.
    • Incubate cells with primary antibody
      • rabbit anti-Myc antibody (antibodies-online, ABIN1532205, lot 424170020 or lot 210020) diluted 1:50 in PBS and mouse anti-HA tag antibody (clone 12CA5) ON at 4°C (images G, H).
      • rabbit anti-Myc antibody (antibodies-online, ABIN1532205, lot 210020 or lot 210020) diluted 1:50 in PBS ON at 4°C (images A, D).
    • Wash cells with 50mM Tris-HCl, pH7.4, 150mM NaCl, 0.1% Tween 20 (TBST) for 15min at RT.
    • Incubate cells with secondary antibody
      • goat anti-rabbit IgG MFP590 (Mobitec MFP-A1037, lot 3002088) diluted 1:300 in PBS and goat anti-mouse IgGDylight488 (Thermo Scientific, 35503) diluted 1:10000 in PBS for 1h at 37°C (images G, H).
      • F(ab')2-Goat anti-Rabbit IgG (H+L) Alexa Fluor 555 (Life Technologies, A21430, lot 1637266) diluted 1:2000 in PBS for 45min at 37°C (images A, D).
    • Counterstain cells with DAPI (Sigma, D-9542).
    • Image acquisition on Zeiss LSM 510 confocal microscope and processing using Adobe Photoshop 5.0.
    Notes

    The antibody detects Myc proteins by immunoblotting and by immunocytology. No cross-reaction with the C-terminal Myc-tag.

  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Stable at -20°C for at least 1 year.
    Date de péremption
    12 months
  • Antigène
    c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
    Autre désignation
    Myc Proto-Oncogene protein (MYC Produits)
    Synonymes
    anticorps MRTL, anticorps MYCC, anticorps bHLHe39, anticorps c-Myc, anticorps CMYC, anticorps c-myc, anticorps C-MYC, anticorps LOC100136746, anticorps ARC, anticorps B430311C09Rik, anticorps MYC, anticorps NOP, anticorps Nop30, anticorps RNCMYC, anticorps mMyc, anticorps AU016757, anticorps Myc2, anticorps Niard, anticorps Nird, anticorps cmyc, anticorps zc-myc, anticorps c-myc II, anticorps myc-B, anticorps myc2, anticorps myc1, anticorps CMyc, anticorps zgc:55680, anticorps CG10798, anticorps D-Myc, anticorps DM, anticorps DMYc, anticorps Dm, anticorps Dmel\CG10798, anticorps Dmyc, anticorps EG:BACN5I9.1, anticorps anon-WO03040301.171, anticorps bHLHe57, anticorps c-MYC, anticorps d-myc, anticorps dMYC, anticorps dMyc, anticorps dMyc1, anticorps da, anticorps dm, anticorps dm/dMyc, anticorps dm/myc, anticorps dmyc, anticorps dmyc1, anticorps l(1)G0139, anticorps l(1)G0354, anticorps l(1)G0359, anticorps myc, anticorps MYC proto-oncogene, bHLH transcription factor, anticorps v-myc myelocytomatosis viral oncogene homolog, anticorps nucleolar protein 3 (apoptosis repressor with CARD domain), anticorps myelocytomatosis oncogene, anticorps MYC proto-oncogene, bHLH transcription factor a, anticorps MYC proto-oncogene, bHLH transcription factor L homeolog, anticorps MYC proto-oncogene, bHLH transcription factor S homeolog, anticorps v-myc myelocytomatosis viral oncogene homolog (avian), anticorps MYC proto-oncogene, bHLH transcription factor b, anticorps CG10798 gene product from transcript CG10798-RB, anticorps MYC, anticorps LOC100136746, anticorps Nol3, anticorps Myc, anticorps myca, anticorps myc.L, anticorps myc.S, anticorps mycb
    Sujet

    Synonyms: Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64 , MYC , BHLHE39

    NCBI Gene Symbol: MYC

    Poids moléculaire
    48 kDa
    ID gène
    4609
    OMIM
    113970
    UniProt
    P01106
    Pathways
    Signalisation p53, Cycle Cellulaire, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, L'effet Warburg
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