beta Actin anticorps (Actin, beta) (N-Term)

Details for Product anti-ACTB Antibody No. ABIN153386
Antigène
  • BRWS1
  • PS1TP5BP1
  • ACTA1
  • Actx
  • E430023M04Rik
  • beta-actin
  • ps1tp5bp1
  • act-2
  • actb
  • B-actin
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • ACT-5
  • ACTB
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • Beta-actin
  • actin beta
  • actin, beta
  • cytoplasmic actin OlCA1
  • beta-actin
  • Actin 5C
  • actin gamma 1
  • actin, beta 1
  • actin, beta L homeolog
  • beta actin
  • actin, cytoplasmic 1
  • ACTB
  • Actb
  • actb
  • LOC100174883
  • LOC100304816
  • Act5C
  • ACTG1
  • actb1
  • actb.L
  • LOC100136352
  • LOC109073280
Épitope
N-Term
119
40
17
13
11
5
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Chat, Poulet, Boeuf (Vache), Chien, Poisson, Cobaye, Hamster, Humain, Sangsue, Mammifères, Souris, Porc, Primate, Lapin, Rat (Rattus), Mouton, Écureuil, Poisson zèbre (Danio rerio)
512
308
270
96
86
67
59
54
48
45
33
32
18
13
13
11
10
10
9
6
6
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Hôte
Souris
326
224
16
5
2
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp beta Actin est non-conjugé
29
27
14
7
7
7
7
7
7
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
482
220
148
103
76
67
35
34
19
14
13
12
12
9
2
1
1
1
1
Options
Immunogène slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Clone AC-15
Isotype IgG1
Specificité In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.
Purification Unpurified
Plasmids, Primers & others Plasmids, Primers & others beta Actin products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation beta-Actin (ACTB Antibody Extrait)
Sujet Gene Symbol: ACTB
Poids moléculaire Theoretical MW: 42 kDa
ID gène 60
Pathways Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
Indications d'application Western Blot 1:5000 - 1:10000, Simple Western 1:25, Flow Cytometry, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:500This antibody is useful for Western blot, ELISA, immunohistochemistry and Immunocytochemistry. The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. This antibody is resistant to formalin fixation and paraffin embedding. B5, methacarn, ethanol or Bouin's solutions may also be used as fixatives. Use in immunoprecipitation reported in scientific literature (PMID 23647384). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size: Wes/Sally Sue/Peggy Sue.
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocole Protocol specific for Beta Actin Antibody IHC-FFPE sections:I. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Buffer Ascites
Buffer contains: 15 mM Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock 4 °C,-20 °C
Stockage commentaire Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Images (Fournisseur)
Image no. 1 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Flow Cytometry: beta-Actin Antibody (AC-15) [ABIN153386] - analysis of HeLa cells usi...
Image no. 2 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Immunohistochemistry: beta-Actin Antibody (AC-15) [ABIN153386] - Breast, Epithelium 40x.
Image no. 3 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) anti-Actin, beta (ACTB) (N-Term) antibody (Image 3)
Image no. 4 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) anti-Actin, beta (ACTB) (N-Term) antibody (Image 4)
Image no. 5 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) anti-Actin, beta (ACTB) (N-Term) antibody (Image 5)
Image no. 6 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - beta-Actin expression in Fib...
Image no. 7 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - MCDK cells induced with doxy...
Image no. 8 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - beta-Actin expression in hum...
Image no. 9 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - beta-Actin expression in CHO...
Image no. 10 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Simple Western: beta-Actin Antibody (AC-15) [ABIN153386] - Analysis using the HRP con...
Image no. 11 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - Analysis of beta-Actin in bo...
Image no. 12 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Flow Cytometry: beta-Actin Antibody (AC-15) [ABIN153386] - Analysis using the HRP con...
Image no. 13 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - beta-Actin expression in hum...
Image no. 14 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Western Blot: beta-Actin Antibody (AC-15) [ABIN153386] - Analysis of beta-actin in NI...
Image no. 15 for anti-Actin, beta (ACTB) (N-Term) antibody (ABIN153386) Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [ABIN153386] - HS...
Produit citée dans: Hughes, Crofton, OBuckley, Herman, Morrow: "Chronic ethanol exposure alters prelimbic prefrontal cortical Fast-Spiking and Martinotti interneuron function with differential sex specificity in rat brain." dans: Neuropharmacology, Vol. 162, pp. 107805, 2020 (PubMed). Méthode utilisée: Western Blotting (WB), Western Blotting (WB) (Échantillon (espèces): Rat (Rattus)).

Xu, Xu, Zhu, Sun, Juguilon, Li, Fan, Yin, Zhang: "Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis." dans: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 28, Issue 1, pp. 202-216, 2020 (PubMed).

Arima, Sasaki, Lee, Zhang, Figueiredo, Mlynarchik, Qiao, Yamada, Higashi, Ha, Halu, Mizuno, Singh, Yamazaki, Aikawa: "Multiorgan Systems Study Reveals Igfbp7 as a Suppressor of Gluconeogenesis after Gastric Bypass Surgery." dans: Journal of proteome research, Vol. 19, Issue 1, pp. 129-143, 2020 (PubMed). (Échantillon (espèces): Mouse (Murine)).

Cheng, Kao, Lee: "Ferulic Acid Exerts Anti-apoptotic Effects against Ischemic Injury by Activating HSP70/Bcl-2- and HSP70/Autophagy-Mediated Signaling after Permanent Focal Cerebral Ischemia in Rats." dans: The American journal of Chinese medicine, Vol. 47, Issue 1, pp. 39-61, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Rat (Rattus)).

Even, Reidenbach, Schlechter, Berns, Herold, Roth, Krunic, Riechmann, Hofmann: "DLIC1, but not DLIC2, is upregulated in colon cancer and this contributes to proliferative overgrowth and migratory characteristics of cancer cells." dans: The FEBS journal, Vol. 286, Issue 4, pp. 803-820, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Chen, Yang, Cheng, Hsiao, Chang, Leu, Wang, Hsu, Huang, Lai: "Modified Sijunzi decoction can alleviate cisplatin-induced toxicity and prolong the survival time of cachectic mice by recovering muscle atrophy." dans: Journal of ethnopharmacology, Vol. 233, pp. 47-55, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Mouse (Murine)).

Lee, Yang, Chen, Tsai, Hung, Chen, Chiang, Chang, Lin: "Novel STAT3 Inhibitor LDOC1 Targets Phospho-JAK2 for Degradation by Interacting with LNX1 and Regulates the Aggressiveness of Lung Cancer." dans: Cancers, Vol. 11, Issue 1, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Davis, Collier, Goodwin, Lukins, Powell, Pennypacker: "Efficacy of leukemia inhibitory factor as a therapeutic for permanent large vessel stroke differs among aged male and female rats." dans: Brain research, Vol. 1707, pp. 62-73, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Rat (Rattus)).

Davis, Collier, Winford, Leonardo, Ajmo, Foran, Kopper, Gensel, Pennypacker: "Leukemia inhibitory factor modulates the peripheral immune response in a rat model of emergent large vessel occlusion." dans: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 288, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Rat (Rattus)).

Zhang, Wei, Xiang, Zhou, Chen, Li, Li, Xiong, Liu: "Semaphorin 4A acts in a feed-forward loop with NF-κB pathway to exacerbate catabolic effect of IL-1β on chondrocytes." dans: International immunopharmacology, Vol. 69, pp. 88-94, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Mouse (Murine)).

Harun-Or-Rashid, Inman: "Reduced AMPK activation and increased HCAR activation drive anti-inflammatory response and neuroprotection in glaucoma." dans: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 313, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Mouse (Murine)).

Spence, Dube, Uezu, Locke, Soderblom, Soderling: "In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation." dans: Nature communications, Vol. 10, Issue 1, pp. 386, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Halu, Wang, Iwata, Mojcher, Abib, Singh, Aikawa, Sharma: "Context-enriched interactome powered by proteomics helps the identification of novel regulators of macrophage activation." dans: eLife, Vol. 7, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Oh, Sung, Kim, Sim, Lee, Baek, Pack, Seok, Seo, Lee, Kim: "Novel Compound Heterozygote Mutation in IL10RA in a Patient With Very Early-Onset Inflammatory Bowel Disease." dans: Inflammatory bowel diseases, Vol. 25, Issue 3, pp. 498-509, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

van der Waals, Laoukili, Jongen, Raats, Borel Rinkes, Kranenburg: "Differential anti-tumour effects of MTH1 inhibitors in patient-derived 3D colorectal cancer cultures." dans: Scientific reports, Vol. 9, Issue 1, pp. 819, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Wang, Pan, Yu, Xiong, Zhao, Xiong, Guo, Lin, Sun, Zhao, Guo, Mei, Xiong: "Lack of Myosin X Enhances Osteoclastogenesis and Increases Cell Surface Unc5b in Osteoclast-Lineage Cells." dans: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, pp. e3667, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Mouse (Murine)).

Ye, Onodera, Blanchard, Kusminski, Esser, Brekken, Scherer: "β1 Syntrophin Supports Autophagy Initiation and Protects against Cerulein-Induced Acute Pancreatitis." dans: The American journal of pathology, Vol. 189, Issue 4, pp. 813-825, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Mouse (Murine)).

Mega Tiber, Kocyigit Sevinc, Kilinc, Orun: "Biological effects of whole Z.Officinale extract on chronic myeloid leukemia cell line K562." dans: Gene, Vol. 692, pp. 217-222, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Di, Lomeli, Bota, Das: "Magmas inhibition as a potential treatment strategy in malignant glioma." dans: Journal of neuro-oncology, Vol. 141, Issue 2, pp. 267-276, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Sakai, Ohhata, Kitagawa, Uchida, Aoshima, Niida, Suzuki, Inoue, Miyazawa, Kitagawa: "Long Noncoding RNA ELIT-1 Acts as a Smad3 Cofactor to Facilitate TGFβ/Smad Signaling and Promote Epithelial-Mesenchymal Transition." dans: Cancer research, Vol. 79, Issue 11, pp. 2821-2838, 2019 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

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