Actin, gamma 1 (ACTG1) (N-Term) anticorps

Détails pour le produit réf. ABIN153419
Antigène
  • ACT
  • ACTG
  • BRWS2
  • DFNA20
  • DFNA26
  • ACTG1
  • ACTA1
  • actg1a
  • Actg
  • actg1b
  • AL023024
  • Actl
  • E51
  • actin
  • cytoplasmic
  • actin gamma 1
  • actin, gamma 1 a
  • actin, gamma 1
  • actin gamma 1 S homeolog
  • actin, gamma, cytoplasmic 1
  • Actin, gamma 1
  • ACTG1
  • actg1
  • Actg1
  • actg1.S
Épitope
N-Term
11
9
5
4
4
4
2
2
1
1
1
1
Reactivité
Humain, Souris
82
37
28
7
5
4
3
2
2
1
1
1
1
1
Hôte
Lapin
65
16
2
1
Clonalité
Polyclonal
Conjugué
Inconjugué
4
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
71
35
23
19
14
11
7
4
4
4
1
1
1
1
Options
Immunogène The epitope recognized by this antibody maps to the N-terminus of human Actin Gamma 1 [UniProt# P63261]. The N-terminus of Actin Gamma 1 is highly conserved with Beta Actin and preliminary indications are that NB600-533 also recognizes Beta Actin (GeneID 60).
Purification Immunogen affinity purified
Antigène
Autre désignation Actin gamma 1 (ACTG1 Antibody Extrait)
Sujet Gene Symbol: ACTG1
Poids moléculaire Theoretical MW: 45 kDa
ID gène 71
UniProt P63261
Pathways Myometrial Relaxation and Contraction, Cell-Cell Junction Organization
Indications d'application Western Blot 1:5000-1:15000, Simple Western 1:100, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:10-1:500This Actin Gamma 1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocole Protocol specific for Actin Gamma 1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 20 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour.
. Dilute the rabbit anti-actin primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Do not freeze.
Stock 4 °C
Stockage commentaire Store at 4°C. Do not freeze.
Images (Fournisseur)
Western Blotting (WB) image for anti-Actin, gamma 1 (ACTG1) (N-Term) antibody (ABIN153419) anti-Actin, gamma 1 (ACTG1) (N-Term) antibody
Western Blotting (WB) image for anti-Actin, gamma 1 (ACTG1) (N-Term) antibody (ABIN153419) Western Blot: Actin Gamma 1 Antibody [ABIN153419] - Whole cell lysate from HeLa (1, 2...
Simple Western (SimWes) image for anti-Actin, gamma 1 (ACTG1) (N-Term) antibody (ABIN153419) Simple Western: Actin Gamma 1 Antibody [ABIN153419] - Simple Western lane view shows ...
Immunofluorescence (IF) image for anti-Actin, gamma 1 (ACTG1) (N-Term) antibody (ABIN153419) Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody [ABIN153419] - Detecti...
Immunocytochemistry (ICC) image for anti-Actin, gamma 1 (ACTG1) (N-Term) antibody (ABIN153419) Immunohistochemistry: Actin Gamma 1 Antibody - Sample: FFPE section of human testicu...
Produit citée dans: Flanagan, Alspach, Pazolli, Parajuli, Ren, Arthur, Tapia, Stewart: "c-Myb and C/EBPβ regulate OPN and other senescence-associated secretory phenotype factors." dans: Oncotarget, Vol. 9, Issue 1, pp. 21-36, 2018 (PubMed).

Luo, Yao, Zhang, Li: "Liquid chromatography-mass spectrometry-based quantitative proteomics analysis reveals chondroprotective effects of astragaloside IV in interleukin-1β-induced SW1353 chondrocyte-like cells." dans: Biomedicine & pharmacotherapy, Vol. 91, pp. 796-802, 2017 (PubMed). Méthode utilisée: Flow Cytometry (FACS), Western Blotting (WB) (Échantillon (espèces): Human).

Blondeau, Lucier, Matteau, Dumont, Rodrigue, Jacques, Blouin: "Dual leucine zipper kinase regulates expression of axon guidance genes in mouse neuronal cells." dans: Neural development, Vol. 11, Issue 1, pp. 13, 2016 (PubMed).

Duxin, Moore, Sidorova, Karanja, Honaker, Dao, Piwnica-Worms, Campbell, Monnat, Stewart: "Okazaki fragment processing-independent role for human Dna2 enzyme during DNA replication." dans: The Journal of biological chemistry, Vol. 287, Issue 26, pp. 21980-91, 2012 (PubMed). (Échantillon (espèces): Human). Détails: Western Blotting

Duxin, Dao, Martinsson, Rajala, Guittat, Campbell, Spelbrink, Stewart: "Human Dna2 is a nuclear and mitochondrial DNA maintenance protein." dans: Molecular and cellular biology, Vol. 29, Issue 15, pp. 4274-82, 2009 (PubMed).

Watters, Kraja, Meucci, Province, McLeod: "Genome-wide discovery of loci influencing chemotherapy cytotoxicity." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 32, pp. 11809-14, 2004 (PubMed).

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