This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogène
This POLR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1437-1465 amino acids from the C-terminal region of human POLR1A.
anticorps 194kDa, anticorps 3010014K16Rik, anticorps RPA194, anticorps Rpo1-4, anticorps mRPA1, anticorps Rpa1, anticorps A190, anticorps RPA1, anticorps RPO1-4, anticorps RPO14, anticorps polymerase (RNA) I polypeptide A, anticorps RNA polymerase I subunit A, anticorps Polr1a, anticorps POLR1A
Sujet
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).