HA-Tag anticorps

Détails pour le produit réf. ABIN1573873
Antigène
Reactivité
Tag
377
5
4
2
1
1
Hôte
Chèvre
193
188
28
17
3
1
Clonalité
Polyclonal
Conjugué
Inconjugué
37
26
16
8
5
5
5
5
5
5
5
5
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
336
159
119
66
44
27
26
15
14
13
11
11
7
6
6
6
5
4
4
4
4
3
2
1
1
1
1
1
Options
Immunogène YPYDVPDYA (influenza hemagglutinin-HA (epitope)) conjugated - KLH
Séquence YPYDVPDYA
Isotype IgG
Specificité This antibody specifically reacts with fusion proteins containing C-terminal, N-terminal, and internal HA tagged fusion proteins.
Purification Immunoaffinity chromatography
Antigène
Classe de substances Tag
Sujet Goat Anti-HA-tag Polyclonal Antibody is highly purified from goat antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% Sodium azide.
Indications d'application Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.5 µg/mL
Western blot: 0.1-1.0 µg/mLImmunofluorescence (IF): 2-10 µg/mLImmunoprecipitation (IP): 2-10 µg/mg of lysateOther applications: user-optimized
Restrictions For Research Use only
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Stock 4 °C/-20 °C
Stockage commentaire The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Images (Fournisseur)
Western Blotting (WB) image for anti-HA-Tag antibody (ABIN1573873) Western blot analysis of HA tagged fusion protein using 1 µg/mL Goat Anti-HA-tag Poly...
Western Blotting (WB) image for anti-HA-Tag antibody (ABIN1573873) Western blot analysis of HA tagged fusion protein using 1 µg/mL Goat Anti-HA-tag Poly...
Western Blotting (WB) image for anti-HA-Tag antibody (ABIN1573873) Western blot analysis of immunoprecipitates from HA fusion protein lysates using Goat...
Produit citée dans: Jiang, Zhao, Lu, Wang, Chen, Tao, Liu, Sun, Zhang, Ma: "Piwil2 inhibits keratin 8 degradation through promoting p38-induced phosphorylation to resist Fas-mediated apoptosis." dans: Molecular and cellular biology, Vol. 34, Issue 21, pp. 3928-38, 2014 (PubMed).

Jennelle, Hunegnaw, Dubrovsky, Pushkarsky, Fitzgerald, Sviridov, Popratiloff, Brichacek, Bukrinsky: "HIV-1 protein Nef inhibits activity of ATP-binding cassette transporter A1 by targeting endoplasmic reticulum chaperone calnexin." dans: The Journal of biological chemistry, Vol. 289, Issue 42, pp. 28870-84, 2014 (PubMed).

Lee, Sethi, Leonardi, Rana, Buettner, Haltiwanger, Bakker, Jafar-Nejad: "Negative regulation of notch signaling by xylose." dans: PLoS genetics, Vol. 9, Issue 6, pp. e1003547, 2013 (PubMed).

Wu, Sapkota, Li, Mu: "Onecut 1 and Onecut 2 are potential regulators of mouse retinal development." dans: The Journal of comparative neurology, Vol. 520, Issue 5, pp. 952-69, 2012 (PubMed).

Ghoujal, Milev, Ajamian, Abel, Mouland: "ESCRT-II's involvement in HIV-1 genomic RNA trafficking and assembly." dans: Biology of the cell / under the auspices of the European Cell Biology Organization, Vol. 104, Issue 12, pp. 706-21, 2012 (PubMed).

Chandrasekharan, Huang, Sun: "Decoding the trans-histone crosstalk: methods to analyze H2B ubiquitination, H3 methylation and their regulatory factors." dans: Methods (San Diego, Calif.), Vol. 54, Issue 3, pp. 304-14, 2011 (PubMed).

Anantharam, Bittner, Aikman, Stuenkel, Schmid, Axelrod, Holz: "A new role for the dynamin GTPase in the regulation of fusion pore expansion." dans: Molecular biology of the cell, Vol. 22, Issue 11, pp. 1907-18, 2011 (PubMed).

Li, Yeh, Chiu, Tang, Tu: "Hyperphosphorylation as a defense mechanism to reduce TDP-43 aggregation." dans: PLoS ONE, Vol. 6, Issue 8, pp. e23075, 2011 (PubMed).

Chapdelaine, Pichavant, Rousseau, Pâques, Tremblay: "Meganucleases can restore the reading frame of a mutated dystrophin." dans: Gene therapy, Vol. 17, Issue 7, pp. 846-58, 2010 (PubMed).

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