HAT-Tag anticorps

N° du produit ABIN1573907

Détail du produit

Antigène: HAT-Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB)

Séquence: KDHLIHNVHK EFHAHAHNK

Specificité: Rabbit Anti-HAT-tag Polyclonal Antibody specifically recognizes C-terminal, N-terminal, and internal HAT tagged fusion proteins.

Purification: Immunoaffinity chromatography

Immunogène: HAT epitope tag peptide KDHLIHNVHKEFHAHAHNK conjugated - KLH

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 5 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS, pH7.4, containing 0.02 % sodium azid

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Référence

Chandrasekera, Wan, Gizewski, Auchampach, Lasley: "Adenosine A1 receptors heterodimerize with ?1- and ?2-adrenergic receptors creating novel receptor complexes with altered G protein coupling and signaling." dans: Cellular signalling, Vol. 25, Issue 4, pp. 736-42, (2013) (PubMed).

Okada, Yamaura, Suzuki, Itoh, Osada, Takahashi: "Molecular characterization and expression analysis of chitinase from the Pacific oyster Crassostrea gigas." dans: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Vol. 165, Issue 2, pp. 83-9, (2013) (PubMed).

Xiao, Zhou, Sun, Feng, Du, Gao, Ji, Yang, Wang, Han, Langford, Lei: "Apa is a trimeric autotransporter adhesin of Actinobacillus pleuropneumoniae responsible for autoagglutination and host cell adherence." dans: Journal of basic microbiology, Vol. 52, Issue 5, pp. 598-607, (2012) (PubMed).

Détail du antigène

Antigène: HAT-Tag

Classe de substances: Tag

Sujet: Rabbit Anti-HAT-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% Sodium azide.