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RFP anticorps

RFP Reactivité: Discosoma WB, ELISA Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1573924
  • Antigène Voir toutes RFP Anticorps
    RFP (Red Fluorescent Protein (RFP))
    Reactivité
    • 53
    • 1
    Discosoma
    Hôte
    • 23
    • 15
    • 9
    • 5
    • 3
    Lapin
    Clonalité
    • 32
    • 23
    Polyclonal
    Conjugué
    • 21
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp RFP est non-conjugé
    Application
    • 29
    • 22
    • 15
    • 12
    • 10
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Fonction
    RFP-tag Antibody, pAb, Rabbit
    Séquence
    VNGHEFEIEGEGEGR
    Specificité
    Rabbit Anti-RFP-tag Polyclonal Antibody specifically reacts with fusion proteins containing RFP-tags. The antibody does not react with GFP or GFP-tagged proteins.
    Réactivité croisée (Details)
    RFP-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing RFP-tags and RFP variants, such as mCherry, tdTomato, DsRed, DsRed2, mOrange. The antibody does not react with GFP or GFP-tagged proteins.
    Purification
    Immunoaffinity chromatography
    Immunogène
    RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated - KLH
    Isotype
    IgG
    Featured
    Discover our best selling RFP Anticorps primaire
    Top Product
    Discover our top product RFP Anticorps primaire
  • Indications d'application
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.05-0.2 µg/mL
    Western blot: 0.1-1.0 µg/mL
    Western blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing cycles.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
  • Alvarez-Guaita, Vilà de Muga, Owen, Williamson, Magenau, García-Melero, Reverter, Hoque, Cairns, Cornely, Tebar, Grewal, Gaus, Ayala-Sanmartín, Enrich, Rentero: "Evidence for annexin A6-dependent plasma membrane remodelling of lipid domains." dans: British journal of pharmacology, Vol. 172, Issue 7, pp. 1677-90, (2015) (PubMed).

    Yang, Shen, Garre, Hao, Krumlinde, Cvijovi?, Arens, Nyström, Liu, Sunnerhagen: "Stress granule-defective mutants deregulate stress responsive transcripts." dans: PLoS genetics, Vol. 10, Issue 11, pp. e1004763, (2014) (PubMed).

    Devers, Teply, Reinert, Gaude, Krajinski: "An endogenous artificial microRNA system for unraveling the function of root endosymbioses related genes in Medicago truncatula." dans: BMC plant biology, Vol. 13, pp. 82, (2013) (PubMed).

    Roberts, Baines: "Myosin Va enhances secretion of herpes simplex virus 1 virions and cell surface expression of viral glycoproteins." dans: Journal of virology, Vol. 84, Issue 19, pp. 9889-96, (2010) (PubMed).

    Nilsson, Sunnerhagen: "Cellular stress induces cytoplasmic RNA granules in fission yeast." dans: RNA (New York, N.Y.), Vol. 17, Issue 1, pp. 120-33, (2010) (PubMed).

  • Antigène
    RFP (Red Fluorescent Protein (RFP))
    Autre désignation
    RFP-Tag (RFP Produits)
    Sujet
    Well-characterizated antibodies against short-sequence epitope tags are common in the study of protein expression in systems. RFP-tags are composed of 15-residue peptides, VNGHEFEIEGEGEGR, derived from amino acids 22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone.
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