SRT Tag anticorps

Détails pour le produit réf. ABIN1573939
Antigène
Reactivité
Tag
2
Hôte
Lapin
12
Clonalité
Polyclonal
Conjugué
Inconjugué
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
5
1
Options
Immunogène Rabbit anti-SRT tag is a synthetic SRT tag peptide with the sequence TFIGAIATDT, conjugated - KLH
Séquence TFIGAIATDT
Isotype IgG
Specificité This Antibody recognizes both N-terminal and C-terminal SRT tagged fusion proteins.
Purification Immunoaffinity chromatography
Antigène
Autre désignation SRT-Tag
Classe de substances Tag
Sujet Well-characterized antibodies for epitope tags consisting of short sequences are often used in the study of protein expression in various systems. The SRT-tag is a peptide epitope comprised of 10 amino acids derived from the crystalline surface layer protein of Rickettsia typhi1.Rabbit Anti-SRT-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic SRT tag peptide conjugated to KLH.Rabbit Anti-SRT-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide.
Indications d'application Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized
Restrictions For Research Use only
Format Liquid
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Stock 4 °C/-20 °C
Stockage commentaire The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Images (Fournisseur)
 image for anti-SRT Tag antibody (ABIN1573939) Lane 1-2: SRT-tag fusion protein in E. coli cell lysate (~ 52 kDa) Lane 3: Negative E...
Western Blotting (WB) image for anti-SRT Tag antibody (ABIN1573939) Loading: SRT-tag fusion protein expressed in E. coli cell lysateAntibody: 1 µg/mL Rab...
Western Blotting (WB) image for anti-SRT Tag antibody (ABIN1573939) Western blot analysis of SRT tagged fusion proteins expressed in E. coli cell lysate ...
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