Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized