tau anticorps (Ser422)

N° du produit ABIN1574008

Détail du produit anti-tau anticorps

Antigène: tau (tau Protein)

Épitope: Ser422

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp tau est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Rabbit Anti-Tau (Ser422) Polyclonal Antibody detects human tau protein, not yet tested for other species.

Réactivité croisée (Details): Reactivity to other species is not tested yet.

Purification: Immunoaffinity chromatography

Immunogène: Synthetic peptide (KLH-coupled) corresponding - AA surrounding Ser422 of human tau

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-2 µg/mL
Other applications: user optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Références pour anticorps anti-tau (ABIN1574008)

Zheng, Amin, Hu, Rudrabhatla, Shukla, Kanungo, Kesavapany, Grant, Albers, Pant: "A 24-residue peptide (p5), derived from p35, the Cdk5 neuronal activator, specifically inhibits Cdk5-p25 hyperactivity and tau hyperphosphorylation." dans: The Journal of biological chemistry, Vol. 285, Issue 44, pp. 34202-12, (2010) (PubMed).

Détails sur tau

Antigène: tau (tau Protein)

Autre désignation: tau (tau Produits)

Synonymes: anticorps DDPAC, anticorps FTDP-17, anticorps MAPTL, anticorps MSTD, anticorps MTBT1, anticorps MTBT2, anticorps PPND, anticorps TAU, anticorps tau, anticorps PHF-tau, anticorps microtubule associated protein tau, anticorps MAPT

Sujet: Tau is a microtubule-associated phosphoprotein (MAP), localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms, which range in size from 352 to 441 amino acids. The tau variants differ from each other by the presence of either three or four repeat-regions in the carboxy-terminal part of the molecule and the absence or presence of one or two inserts in the amino-terminal portion. Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3beta transfection phosphorylates serines 199, 202, 235, 396, 404, and 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of tau. Phosphorylation on these sites reduces the ability of a given tau species to promote microtubule self-assembly. Hyperphosphorylated tau is the major protein of the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer-disease (AD). The PHFs are also found in the lesions of other central nervous system disorders. Rabbit Anti-Tau (Ser422) Polyclonal Antibody is developed in rabbit using a synthetic peptide (KLH-coupled) corresponding to residues surrounding serine 422 of human tau.