Cofilin 1 (CFL1) (N-Term) anticorps

N° du produit ABIN1574056

Détail du produit

Antigène:

Cofilin 1 (CFL1)

Épitope: N-Term

Reactivité: Cynomolgus, Humain, Souris, Porc, Rat, Mouton

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Specificité: Rabbit Anti-Cofilin Polyclonal Antibody detects endogenous levels of human and mouse cofilin protein. It is predicted to react with cynomolgus monkey, sheep, pig, and rat cofilin protein according to sequence homology.

Réactivité croisée (Details): Rabbit Anti-Cofilin Polyclonal Antibody detects endogenous levels of human and mouse cofilin protein. It is predicted to react with cynomolgus monkey, sheep, pig, and rat cofilin protein according to sequence homology.

Purification: Immunoaffinity chromatography

Immunogène: Synthetic peptide (KLH-coupled) from amino terminal of human cofilin protein .

Isotype: IgG1

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunohistochemistry: 5-10 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène:

Cofilin 1 (CFL1)

Autre désignation: Cofilin (CFL1 Antibody Extrait)

Sujet: Cofilin belongs to the actin-binding protein ADF family and it contains 1 ADF-H domain. Cofilin controls reversible actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin at a 1:1 ratio. It is the major component of intranuclear and cytoplasmic actin rods. It is involved in the translocation of the actin-cofilin complex from cytoplasm to nucleus. This protein is widely distributed in various tissues.Rabbit Anti-Cofilin Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide from amino terminal of human cofilin protein (Swiss Prot: P23528).

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events