Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

DARPP32 anticorps (AA 150-200)

PPP1R1B Reactivité: Humain, Souris, Rat WB, FACS Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1574114
  • Antigène Voir toutes DARPP32 (PPP1R1B) Anticorps
    DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))
    Épitope
    • 33
    • 17
    • 15
    • 9
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 150-200
    Reactivité
    • 95
    • 83
    • 75
    • 13
    • 12
    • 12
    • 7
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 108
    • 12
    • 1
    • 1
    • 1
    • 1
    Lapin
    Clonalité
    • 102
    • 22
    Polyclonal
    Conjugué
    • 73
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp DARPP32 est non-conjugé
    Application
    • 94
    • 38
    • 35
    • 28
    • 27
    • 18
    • 18
    • 8
    • 5
    • 4
    • 2
    • 1
    Western Blotting (WB), Flow Cytometry (FACS)
    Specificité
    Rabbit Anti-DARPP-32 Polyclonal Antibody detects endogenous levels of mouse and rat DARPP-32. Predicted to react with human DARPP-32 according to sequence homology.Positive Control: Mouse brain and rat brain
    Réactivité croisée (Details)
    Rabbit Anti-DARPP-32 Polyclonal Antibody detects endogenous levels of mouse and rat DARPP-32. Predicted to react with human DARPP-32 according to sequence homology.Positive Control: Mouse brain and rat brain
    Purification
    Immunoaffinity chromatography
    Immunogène
    KLH-coupled synthetic peptide within AA 150-200 of human DARPP-32 .
    Isotype
    IgG
    Top Product
    Discover our top product PPP1R1B Anticorps primaire
  • Indications d'application
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther Applications: user-optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 0.02 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Antigène
    DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))
    Autre désignation
    DARPP-32 (PPP1R1B Produits)
    Synonymes
    anticorps darpp32, anticorps darpp-32, anticorps Darpp-32, anticorps Darpp32, anticorps DARPP-32, anticorps DARPP32, anticorps AU040756, anticorps protein phosphatase 1 regulatory inhibitor subunit 1B, anticorps protein phosphatase 1, regulatory (inhibitor) subunit 1B, anticorps protein phosphatase 1 regulatory inhibitor subunit 1B S homeolog, anticorps ppp1r1b, anticorps PPP1R1B, anticorps Ppp1r1b, anticorps ppp1r1b.S
    Sujet
    DARPP-32 (Dopamine- and cAMP-Regulated Neuronal Phosphoprotein, Mr 32 kDa), also known as PPP1R1B, is expressed in medium-sized spiny neurons that also express dopamine D1 receptors. It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine stimulates phosphorylation of DARPP-32 through dopamine D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1. DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5).Rabbit Anti-DARPP-32 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 150-200 of human DARPP-32 (Swiss Prot: Q9UD71).
Vous êtes ici:
Support technique