TARDBP anticorps

N° du produit ABIN1580460

Détail du produit anti-TARDBP anticorps

Antigène: TARDBP (TAR DNA Binding Protein (TARDBP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TARDBP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: affinity purified antibody

Clone: 3H8

Isotype: IgG1

Information d'application

Indications d'application: The antibody will work at dilutions of 1:5,000 on sections and cells in tissue culture and 1:20,000 on western blots.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4°C short term or -20°C long term.

Détails sur TARDBP

Antigène: TARDBP (TAR DNA Binding Protein (TARDBP))

Autre désignation: Tar DNA binding protein 43 (TARDBP Produits)

Synonymes: anticorps TARDBP, anticorps TDP43, anticorps DKFZp459I2127, anticorps tardbp, anticorps ALS10, anticorps TDP-43, anticorps 1190002A23Rik, anticorps C85084, anticorps Tdp43, anticorps wu:fb77f02, anticorps wu:fc52g10, anticorps TAR DNA binding protein, anticorps Tardbp protein, anticorps TAR DNA binding protein L homeolog, anticorps TAR DNA-binding protein 43-like, anticorps TARDBP, anticorps tardbp, anticorps tardbp.L, anticorps Tardbp, anticorps LOC100621383

Sujet: TDP43 was originally identified as a protein which binds to the transactivation response (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome. UV cross-linking of HeLa cell extract revealed a 43 kDa protein which was cloned and sequenced and shown to contain a two copies of the approx. 90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. It also has a single Zinc Finger domain of the ZnF_RBZ subtype found in Ran binding proteins. Ran is a small G protein related to p21-Ras which regulates the import and export of proteins to the nucleus. The protein is frequently refered to by the acronym TDP43 corresponds to TAR DNA binding protein of molecular weight 43 kDa, although the Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC) name is TARDBP. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis (ALS, 2). The protein is present in these inclusions in a partially degraded, hyperphosphorylated and ubiquitinated form. This antibody has been used in a recent peer reviewed publication, showing that it works well on western blots and on histological sections. Further images of histological staining using this antibody showing clears staining of ALS related inclusion bodies is shown here. The HGNC name for this protein is TARDBP.

Pathways: Positive Regulation of Peptide Hormone Secretion