MYH6 anticorps

N° du produit ABIN1607600

Détail du produit anti-MYH6 anticorps

Antigène: MYH6 (Myosin Heavy Chain 6, Cardiac Muscle, alpha (MYH6))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MYH6 est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity with myosin6 from other sources have not been determined.

Pureté: Anti-Myosin-6 is directed against the human myosin-6 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with human only.

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Anti-Myosin-6 was prepared from whole rabbit serum produced by repeated immunizations with a truncated myosin-6 construct expressed in SF9 insect cells corresponding to human myosyn-6 protein.
Immunogen Type: Recombinant Protein

Information d'application

Indications d'application: Myosin-6 antibody has been tested for use in ELISA and western blot. For western blots expect a band of approximately 150 kDa in size corresponding to truncated myosin-6 protein. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:10.000
Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 25 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur MYH6

Antigène: MYH6 (Myosin Heavy Chain 6, Cardiac Muscle, alpha (MYH6))

Autre désignation: Myosin 6 (MYH6 Produits)

Synonymes: anticorps Myhca, anticorps ASD3, anticorps CMD1EE, anticorps CMH14, anticorps MYHC, anticorps MYHCA, anticorps SSS3, anticorps alpha-MHC, anticorps A830009F23Rik, anticorps AA517445, anticorps Myhc-a, anticorps amhc, anticorps MYH6, anticorps P-MHC, anticorps VMHC1, anticorps myosin, anticorps myosin heavy chain 6, anticorps myosin, heavy polypeptide 6, cardiac muscle, alpha, anticorps myosin, heavy chain 6, cardiac muscle, alpha, anticorps myosin-6, anticorps myosin, heavy chain 15, anticorps Myh6, anticorps MYH6, anticorps LOC101120580, anticorps myh6, anticorps MYH15

Sujet: Myosin VI is a myosin superfamily member with unique and intriguing features that allow it to fill a still-expanding number of cell biological roles. This actin-based motor produces force that acts towards the minus end of actin filaments, which is the opposite direction to all other characterized myosins. In mammalian cells, myosin VI is localized to endocytic vesicles, membrane ruffles, the cytosol and the Golgi complex. Its motor function is essential for several physiological functions of the cell, including normal rates of endocytosis, maintenance of Golgi morphology and protein secretion. Myosin VI regulates epithelial cell migration and plays a role in the maintenance of adhesive cellular contacts within epithelial cell layers. It is highly expressed in ovarian cancers and prostate cancers and its expression level, which is upregulated by DNA damage in a p53-dependent manner, correlates with the potential of the tumor to disseminate. More recently, myosin VI has been found involved in EGFR endocytosis through a clathrin dependent mechanism. Synonyms: MyosinVI, Unconventional myosin 6

ID gène: 3799

NCBI Accession: NP_004512

UniProt: P33176