Lactate Dehydrogenase anticorps (Biotin)

N° du produit ABIN1607671

Détail du produit anti-Lactate Dehydrogenase anticorps (Biotin)

Antigène: Lactate Dehydrogenase (LDH)

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Lactate Dehydrogenase est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Lactate Dehydrogenase from other sources may occur but have not been specifically determined.

Pureté: Anti-lactate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Lactate Dehydrogenase [full length protein isolated from rabbit muscle]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-lactate dehydrogenase antibody has been assayed against 1.0 µg of Lactate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Références pour anticorps anti-Lactate Dehydrogenase (Biotin) (ABIN1607671)

Vincent, Cornea, Jong, Laferrière, Kumar, Mickeviciute, Fung, Bandegi, Ribeiro-da-Silva, OMalley, Coderre: "Intracellular mGluR5 plays a critical role in neuropathic pain." dans: Nature communications, Vol. 7, pp. 10604, (2016) (PubMed).

Détails sur Lactate Dehydrogenase

Antigène: Lactate Dehydrogenase (LDH)

Autre désignation: Lactate Dehydrogenase (LDH Produits)

Synonymes: anticorps CG10160, anticorps Dmel\\CG10160, anticorps IMP-L3, anticorps IMPL3, anticorps Imp-L3, anticorps Impl3, anticorps LDH, anticorps Ld, anticorps Ldh, anticorps dLdha, anticorps dmLDH, anticorps Ecdysone-inducible gene L3, anticorps lactate dehydrogenase, anticorps ldh2, anticorps ImpL3, anticorps ldh, anticorps ldh2, anticorps Ldh, anticorps LDH-A

Sujet: Anti-lactate dehydrogenase recognizes lactate dehydrogenase which catalyzes the interconversion of pyruvate to lactate and NADH to NAD+. This reaction occurs in the absence or lack of oxygen, making lactate dehydrogenase critical for anerobic respiration. There five known isozymes of LDH, each localized in different types of tissue. In medicine, LDH is often used as a marker of various diseases ranging from hemolysis to encephalitis. Anti-lactate dehydrogenase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction. Synonyms: L-lactate dehydrogenase A chain LDH-A LDH muscle subunit LDH-M

ID gène: 100009107, 126050

UniProt: P13491