AKT3 anticorps (Internal Region)

N° du produit ABIN1607681

Détail du produit anti-AKT3 anticorps

Antigène: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Épitope: Internal Region

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AKT3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Réactivité croisée (Details): Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross reactivity with AKT2 and AKT has not been determined.

Pureté: Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography.

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.
Immunogen Type: Peptide

Clone: 25F6-F6-D8

Isotype: IgG1 kappa

Information d'application

Indications d'application: Anti-AKT3 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
ELISA Dilution: 1:2.000 - 1:10.000
Immunohistochemistry Dilution: 20 µg/mL
Western Blot Dilution: 1:500-1:2000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur AKT3

Antigène: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Autre désignation: AKT3 (AKT3 Produits)

Synonymes: anticorps MPPH, anticorps PKB-GAMMA, anticorps PKBG, anticorps PRKBG, anticorps RAC-PK-gamma, anticorps RAC-gamma, anticorps STK-2, anticorps AI851531, anticorps D930002M15Rik, anticorps Nmf350, anticorps Pkbg, anticorps AKT serine/threonine kinase 3, anticorps thymoma viral proto-oncogene 3, anticorps v-akt murine thymoma viral oncogene homolog 3b, anticorps AKT3, anticorps Akt3, anticorps akt3b

Sujet: AKT3 Antibody detects AKT3 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT3 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research. Synonyms: AKT 3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma

ID gène: 10000

UniProt: Q9Y243

Pathways: Signalisation PI3K-Akt, Signalisation RTK, Signalisation TLR, Hepatitis C, VEGF Signaling