ASRGL1 anticorps (Biotin)

N° du produit ABIN1607805

Détail du produit anti-ASRGL1 anticorps (Biotin)

Antigène: ASRGL1 (Asparaginase Like 1 (ASRGL1))

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ASRGL1 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity against Asparaginase from other sources may occur but have not been specifically determined.

Pureté: Anti-L-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Asparaginase [E. coli].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Asparaginase [E. coli]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-L-Asparaginase has been assayed against 1.0 µg of Asparaginase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.200 to 1:12,800 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur ASRGL1

Antigène: ASRGL1 (Asparaginase Like 1 (ASRGL1))

Autre désignation: L-Asparaginase (ASRGL1 Produits)

Synonymes: anticorps ASRGL1, anticorps ALP, anticorps ALP1, anticorps CRASH, anticorps 2410004D18Rik, anticorps AU040643, anticorps AW060375, anticorps Gliap, anticorps Hiob2, anticorps zgc:103568, anticorps asparaginase like 1, anticorps L-asparaginase, anticorps isoaspartyl peptidase/L-asparaginase, anticorps ASRGL1, anticorps STY0886, anticorps ansA, anticorps ECA0132, anticorps LOC5580113, anticorps EcHS_A0886, anticorps LACBIDRAFT_317700, anticorps BCAM1291, anticorps ZPR_0296, anticorps PAAG_01206, anticorps asrgl1, anticorps Asrgl1

Sujet: Anti-L-Asparaginase antibody recognizes the protein asparaginase. Asparaginase is responsible for the catalysis of aspargine to L-aspartic acid and ammonia. In food processing, asaparaginase is used to prevent the formation of acrylamide, a carcinogen, from the heating of asparagine which causes it to undergo the maillard reaction. Asparaginase is also used in cancer therapy since leukemic cells are unable to produce their own asparagine and therefore, must rely on circulating asparagine. Treatment with asparaginase converts the asparagine to aspartic acid denying the leukemic cells of asparagine, resulting in their death. Anti-L-Asparaginase antibody is suitable to investigators interested in cancer, metabolism, and enzymology. Synonyms: L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody

ID gène: 1039460

UniProt: P00805