SNAP alpha/beta anticorps

N° du produit ABIN1742242

Détail du produit anti-SNAP alpha/beta anticorps

Antigène: SNAP alpha/beta

Reactivité: Humain, Rat, Souris, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SNAP alpha/beta est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificité: Specific for alpha- and beta SNAP

Réactivité croisée (Details): does not cross-react to gamma SNAP.

Purification: purified IgG

Immunogène: Recombinant full length rat alpha SNAP.

Clone: 77-2

Isotype: IgG1

Information d'application

Indications d'application: WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC: not tested yet

Commentaires:

The antibody does not immunoprecipitate the 20 S SNARE-complex.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Conseil sur la manipulation: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Stock: -20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Références pour anticorps anti-SNAP alpha/beta (ABIN1742242)

de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." dans: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Hanson, Otto, Barton, Jahn: "The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin." dans: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16955-61, (1995) (PubMed).

Détails sur SNAP alpha/beta

Antigène: SNAP alpha/beta

Abstract: SNAP alpha/beta Produits