UNC13A/Munc13-1 anticorps (AA 3-317)

N° du produit ABIN1742276

Détail du produit anti-UNC13A/Munc13-1 anticorps

Antigène: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Épitope: AA 3-317

Reactivité: Souris, Rat, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp UNC13A/Munc13-1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Specificité: Specific for munc 13-1.

Purification: purified IgG

Immunogène: Recombinant rat munc 13-1 (aa 3-317).

Clone: 266B1

Isotype: IgG2b

Information d'application

Indications d'application: WB: 1 : 500 up to 1 : 5000 (AP staining)
ICC: limited
IHC: not tested yet

Commentaires:

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: limited to methanol fixation. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Conseil sur la manipulation: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Stock: -20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Références pour anticorps anti-UNC13A/Munc13-1 (ABIN1742276)

Kaeser-Woo, Younts, Yang, Zhou, Wu, Castillo, Südhof: "Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 23, pp. 9769-80, (2013) (PubMed).

Dudenhöffer-Pfeifer, Schirra, Pattu, Halimani, Maier-Peuschel, Marshall, Matti, Becherer, Dirks, Jung, Lipp, Hoth, Sester, Krause, Rettig: "Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes." dans: Traffic (Copenhagen, Denmark), Vol. 14, Issue 7, pp. 798-809, (2013) (PubMed).

Maas, Torres, Altrock, Leal-Ortiz, Wagh, Terry-Lorenzo, Fejtova, Gundelfinger, Ziv, Garner: "Formation of Golgi-derived active zone precursor vesicles." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 32, pp. 11095-108, (2012) (PubMed).

Martín, Bartolomé-Martín, Torres, Sánchez-Prieto: "Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals." dans: Journal of neurochemistry, Vol. 116, Issue 4, pp. 476-85, (2011) (PubMed).

Martín, Durroux, Ciruela, Torres, Pin, Sánchez-Prieto: "The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals." dans: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17907-17, (2010) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." dans: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Détails sur UNC13A/Munc13-1

Antigène: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Autre désignation: Munc 13-1 (UNC13A Produits)

Synonymes: anticorps Munc13-1, anticorps Unc13h1, anticorps 2410078G03Rik, anticorps unc-13 homolog A, anticorps unc-13 homolog A (C. elegans), anticorps Unc13a, anticorps UNC13A

Pathways: Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis